User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2012/09/13

Objective

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Description

1. Place culture tubes on ice (6 tubes total for use with different volume of cells and volume of DNA)
   1. 30uL cells (direct from supplier), 10uL of DNA (from Hartings workup)
   2. 30uL cells (direct from supplier), 10uL of DNA (from Costanzi workup)
   3. 30uL cells (made by Tamra), 10uL of DNA (from Hartings workup)
   4. 30uL cells (made by Tamra), 10uL of DNA (from Costanzi workup)
   5. 30uL cells (made by Tamra), 5uL of DNA (from Hartings workup)
   6. 30uL cells (made by Tamra), 5uL of DNA (from Costanzi workup)
2. Thaw cells
3. Add appropriate amount of DNA
4. Add appropriate cells
5. Incubate on ice for 30minutes
6. Heat shock at 42C for 30seconds
7. Place on ice
8. Add 250uL of SOC
9. Shake at 37C for 1 hour
10. Plate cells.

Data

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Notes

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