User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2012/09/13: Difference between revisions
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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span> | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==Objective== | ==Objective== | ||
Transformation from yesterday didn't work. Try again using protocol from invitrogen. | |||
==Description== | ==Description== | ||
# | #Place culture tubes on ice (6 tubes total for use with different volume of cells and volume of DNA) | ||
##30uL cells (direct from supplier), 10uL of DNA (from Hartings workup) | |||
##30uL cells (direct from supplier), 10uL of DNA (from Costanzi workup) | |||
##30uL cells (made by Tamra), 10uL of DNA (from Hartings workup) | |||
##30uL cells (made by Tamra), 10uL of DNA (from Costanzi workup) | |||
##30uL cells (made by Tamra), 5uL of DNA (from Hartings workup) | |||
##30uL cells (made by Tamra), 5uL of DNA (from Costanzi workup) | |||
#Thaw cells | |||
#Add appropriate amount of DNA | |||
#Add appropriate cells | |||
#Incubate on ice for 30minutes | |||
#Heat shock at 42C for 30seconds | |||
#Place on ice | |||
#Add 250uL of SOC | |||
#Shake at 37C for 1 hour | |||
#Plate cells. | |||
==Data== | ==Data== |
Latest revision as of 21:59, 26 September 2017
Biomaterials Design Lab | Main project page Previous entry Next entry |
ObjectiveTransformation from yesterday didn't work. Try again using protocol from invitrogen. Description
Data
NotesThis area is for any observations or conclusions that you would like to note.
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