User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2012/09/17

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(Autocreate 2012/09/17 Entry for User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab)
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(Description)
 
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==Objective==
==Objective==
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Learn how to maintain an OpenWetWare Notebook.
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Prep AuNP samples for AA analysis tomorrow.
==Description==
==Description==
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# Add experimental record here. Include what, how, and why...
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I made two stock solutions
 +
 
 +
Au: 20.3mg of HAuCl<sub>4</sub>·H<sub>2</sub>O in 24mL of water (2.36mM) and
 +
 
 +
BSA: 47.2mg of BSA in 45mL of water (15.7uM).
 +
 
 +
Samples were made in the following manner using similar ratios to the experiments I ran on [[User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2012/05/23|May 23rd]]
 +
 
 +
I made 2 solutions (11.8 mg of HAuCl<sub>4</sub> in 14 mL of water and 23.2 mg of Bovine Serum Albumin in 22 mL of water) and used these solutions to make samples for preparing AuNPs.
 +
{|style="width:700px"
 +
|<u>Approx. Au/BSA ratio</u>
 +
|<u>Volume Au added (mL)</u>
 +
|<u>Volume BSA added (mL)</u>
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|<u>Volume Water added (mL)</u>
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|<u>Actual Ratio</u>
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|-
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|<b>20</b>
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|1.00
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|8.334
 +
|0.666
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|18.0
 +
|-
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|<b>30</b>
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|1.00
 +
|5.554
 +
|3.446
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|27.1
 +
|-
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|<b>40</b>
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|1.00
 +
|4.166
 +
|4.834
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|36.1
 +
|-
 +
|<b>50</b>
 +
|1.00
 +
|3.334
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|5.666
 +
|45.1
 +
|-
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|<b>60</b>
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|1.000
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|2.778
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|6.222
 +
|54.1
 +
|-
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|<b>70</b>
 +
|1.00
 +
|2.38
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|6.62
 +
|63.1
 +
|-
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|<b>80</b>
 +
|1.00
 +
|2.084
 +
|6.916
 +
|72.1
 +
|-
 +
|<b>90</b>
 +
|1.00
 +
|1.854
 +
|7.146
 +
|81.1
 +
|-
 +
|<b>100</b>
 +
|1.00
 +
|1.666
 +
|7.334
 +
|90.2
 +
|-
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|<b>110</b>
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|1.00
 +
|1.514
 +
|7.486
 +
|99.3
 +
|-
 +
|<b>120</b>
 +
|1.00
 +
|1.386
 +
|7.614
 +
|108.5
 +
|-
 +
|<b>130</b>
 +
|1.00
 +
|1.28
 +
|7.72
 +
|117.4
 +
|-
 +
|<b>140</b>
 +
|1.00
 +
|1.188
 +
|7.812
 +
|126.5
 +
|-
 +
|<b>150</b>
 +
|1.00
 +
|1.112
 +
|7.888
 +
|135.2
 +
|-
 +
|<b>160</b>
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|1.000
 +
|1.04
 +
|7.96
 +
|144.5
 +
|-
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|<b>170</b>
 +
|1.00
 +
|0.98
 +
|8.02
 +
|153.4
 +
|-
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}
==Data==
==Data==
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* Add data and results here...
 
==Notes==
==Notes==
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This area is for any observations or conclusions that you would like to note.
 
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Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.
 
[[Category:Course]]
[[Category:Course]]

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Objective

Prep AuNP samples for AA analysis tomorrow.

Description

I made two stock solutions

Au: 20.3mg of HAuCl4·H2O in 24mL of water (2.36mM) and

BSA: 47.2mg of BSA in 45mL of water (15.7uM).

Samples were made in the following manner using similar ratios to the experiments I ran on May 23rd

I made 2 solutions (11.8 mg of HAuCl4 in 14 mL of water and 23.2 mg of Bovine Serum Albumin in 22 mL of water) and used these solutions to make samples for preparing AuNPs.

}

Data

Notes



Approx. Au/BSA ratio Volume Au added (mL) Volume BSA added (mL) Volume Water added (mL) Actual Ratio
20 1.00 8.334 0.666 18.0
30 1.00 5.554 3.446 27.1
40 1.00 4.166 4.834 36.1
50 1.00 3.334 5.666 45.1
60 1.000 2.778 6.222 54.1
70 1.00 2.38 6.62 63.1
80 1.00 2.084 6.916 72.1
90 1.00 1.854 7.146 81.1
100 1.00 1.666 7.334 90.2
110 1.00 1.514 7.486 99.3
120 1.00 1.386 7.614 108.5
130 1.00 1.28 7.72 117.4
140 1.00 1.188 7.812 126.5
150 1.00 1.112 7.888 135.2
160 1.000 1.04 7.96 144.5
170 1.00 0.98 8.02 153.4


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