User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2012/09/17: Difference between revisions
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==Description== | ==Description== | ||
I made two stock solutions | |||
Au: 20.3mg of HAuCl<sub>4</sub>·H<sub>2</sub>O in 24mL of water and | |||
BSA: 47.2mg of BSA in 45mL of water. | |||
Samples were made in the following manner using similar ratios to the experiments I ran on [[User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2012/05/23|May 23rd]] | |||
I made 2 solutions (11.8 mg of HAuCl<sub>4</sub> in 14 mL of water and 23.2 mg of Bovine Serum Albumin in 22 mL of water) and used these solutions to make samples for preparing AuNPs. | |||
{|style="width:700px" | |||
|<u>Approx. Au/BSA ratio</u> | |||
|<u>Volume Au added (mL)</u> | |||
|<u>Volume BSA added (mL)</u> | |||
|<u>Volume Water added (mL)</u> | |||
|<u>Actual Ratio</u> | |||
|- | |||
|<b>20</b> | |||
|1.00 | |||
|8.334 | |||
|0.666 | |||
|? | |||
|- | |||
|<b>30</b> | |||
|1.00 | |||
|5.554 | |||
|3.446 | |||
|? | |||
|- | |||
|<b>40</b> | |||
|1.00 | |||
|4.166 | |||
|4.834 | |||
|? | |||
|- | |||
|<b>50</b> | |||
|1.00 | |||
|3.334 | |||
|5.666 | |||
|? | |||
|- | |||
|<b>60</b> | |||
|1.000 | |||
|2.778 | |||
|6.222 | |||
|? | |||
|- | |||
|<b>70</b> | |||
|1.00 | |||
|2.38 | |||
|6.62 | |||
|? | |||
|- | |||
|<b>80</b> | |||
|1.00 | |||
|2.084 | |||
|6.916 | |||
|? | |||
|- | |||
|<b>90</b> | |||
|1.00 | |||
|1.854 | |||
|7.146 | |||
|? | |||
|- | |||
|<b>100</b> | |||
|1.00 | |||
|1.666 | |||
|7.334 | |||
|? | |||
|- | |||
|<b>110</b> | |||
|1.00 | |||
|1.514 | |||
|7.486 | |||
|? | |||
|- | |||
|<b>120</b> | |||
|1.00 | |||
|1.386 | |||
|7.614 | |||
|? | |||
|- | |||
|<b>130</b> | |||
|1.00 | |||
|1.28 | |||
|7.72 | |||
|? | |||
|- | |||
|<b>140</b> | |||
|1.00 | |||
|1.188 | |||
|7.812 | |||
|? | |||
|- | |||
|<b>150</b> | |||
|1.00 | |||
|1.112 | |||
|7.888 | |||
|? | |||
|- | |||
|<b>160</b> | |||
|1.000 | |||
|1.04 | |||
|7.96 | |||
|? | |||
|- | |||
|<b>170</b> | |||
|1.00 | |||
|0.98 | |||
|8.02 | |||
|? | |||
|- | |||
} | |||
==Data== | ==Data== |
Revision as of 09:15, 17 September 2012
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ObjectivePrep AuNP samples for AA analysis tomorrow. DescriptionI made two stock solutions Au: 20.3mg of HAuCl4·H2O in 24mL of water and BSA: 47.2mg of BSA in 45mL of water. Samples were made in the following manner using similar ratios to the experiments I ran on May 23rd I made 2 solutions (11.8 mg of HAuCl4 in 14 mL of water and 23.2 mg of Bovine Serum Albumin in 22 mL of water) and used these solutions to make samples for preparing AuNPs. }Data
NotesThis area is for any observations or conclusions that you would like to note.
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