User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2013/08/28: Difference between revisions

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==citrate-AuNP==
==citrate-AuNP==
This procedure is used by Dr. Miller in her research lab - [[User:Allison_K._Alix/Notebook/Thesis_Research/2013/07/19 | Allison Alix's notebook]] and is taken from [http://pubs.acs.org/doi/abs/10.1021/ed081p544A this reference].
This procedure is used by Dr. Miller in her research lab - [[User:Allison_K._Alix/Notebook/Thesis_Research/2013/07/19 | Allison Alix's notebook]] and is taken from [http://pubs.acs.org/doi/pdf/10.1021/ac0702084 this reference] and can be analyzed according to [http://pubs.acs.org/doi/suppl/10.1021/ac0702084/suppl_file/ac0702084si20070321_014144.pdf this reference].


# Make 20mL of 1mM HAuCl<sub>4</sub> in a clean beaker
# Take 50mL of the HAuCl<sub>4</sub> solution from the 250mL volumetric flask. Note the concentration
# Heat this solution to boiling while stirring
# Heat this solution to boiling while stirring
# Add 2mL of 1% (w/v) sodium citrate
# Add 3mL of 1% (w/v) sodium citrate
# Observe a purple to red color change
# Boil solution for another 40 minutes
# Cool to room temperature and measure the volume
# Determine the final concentration of gold and citrate


[[Category:Course]]
[[Category:Course]]

Revision as of 08:29, 27 August 2013

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Objective

Synthesize two different sets of gold nanoparticles. In one set, Au3+ is reduced by a protein (bovine serum albumin, BSA) and the synthesized nanoparticle is also surrounded and stabilized by BSA. In the second set, Au3+ is reduced by citrate, and the AuNP is stabilized by citrate in solution. The BSA-AuNPs are purple in color and the citrate-AuNPs are more of a burgundy (reddish) color.

BSA-AuNP

Start by placing all of your materials into a volumetric flask so that you know the exact volumes and exact amounts of what you have added. I will have prepared the initial protein and gold solutions for you (this one time only). In the future, you will be expected to make most of the starting solutions.

This procedure was taken from the following reference and has been used by our previous two Experimental Biological Chemistry groups.

  1. Add 1mL of the (~2.5mM -note the exact concentration) gold (HAuCl4) solution to a 10mL volumetric flask
  2. Add an appropriate amount of BSA solution so that the final concentration of gold is 90X that of BSA
  3. Add deionized water up to 10mL
  4. Transfer solution to a test tube and cap with aluminum foil
  5. Heat in oven at 80C for 3 hours
  6. Transfer solution to a plastic falcon tube (with blue cap)

citrate-AuNP

This procedure is used by Dr. Miller in her research lab - Allison Alix's notebook and is taken from this reference and can be analyzed according to this reference.

  1. Take 50mL of the HAuCl4 solution from the 250mL volumetric flask. Note the concentration
  2. Heat this solution to boiling while stirring
  3. Add 3mL of 1% (w/v) sodium citrate
  4. Boil solution for another 40 minutes
  5. Cool to room temperature and measure the volume
  6. Determine the final concentration of gold and citrate




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