User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2013/09/24: Difference between revisions
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==Description== | ==Description== | ||
The experimental details (pepsin cleavage of hemoglobin) are similar to [http://pubs.acs.org/doi/abs/10.1021/la001164w this reference]. | |||
We will allow the pepsin cleavage of hemoglobin to proceed for two hours with aliquots taken and analyzed every 1/2 hour. We will also run parallel reactions with different concentrations of pepstatin (pepsin inhibitor) present. We will save our aliquots for analysis tomorrow using SDS-PAGE. | |||
# Combine 5 mL of the hemoglobin solution with the appropriate amount of pepsin (the final concentration should be 2 nM). | |||
## Do the same only with an appropriate amount of pepstatin added. (Each group will use a different concentration of pepstatin. We'll want 2 nM, 200 nM, 20uM, 2mM, and 20mM) | |||
==Data== | ==Data== | ||
Revision as of 19:53, 23 September 2013
 Biomaterials Design Lab
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ObjectiveTo observe the catalytic activity of pepsin (in cleaving peptide bonds in hemoglobin) in the presence and absence of pepstatin. This data will be compared with data we take in a later lab on pepsin-AuNPs. DescriptionThe experimental details (pepsin cleavage of hemoglobin) are similar to this reference. We will allow the pepsin cleavage of hemoglobin to proceed for two hours with aliquots taken and analyzed every 1/2 hour. We will also run parallel reactions with different concentrations of pepstatin (pepsin inhibitor) present. We will save our aliquots for analysis tomorrow using SDS-PAGE.
Data
NotesThis area is for any observations or conclusions that you would like to note.
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