User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2013/09/24

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Objective)
(Description)
Line 14: Line 14:
==Description==
==Description==
-
# Add experimental record here. Include what, how, and why...
+
The experimental details (pepsin cleavage of hemoglobin) are similar to [http://pubs.acs.org/doi/abs/10.1021/la001164w this reference].
 +
 
 +
We will allow the pepsin cleavage of hemoglobin to proceed for two hours with aliquots taken and analyzed every 1/2 hour. We will also run parallel reactions with different concentrations of pepstatin (pepsin inhibitor) present. We will save our aliquots for analysis tomorrow using SDS-PAGE.
 +
 
 +
# Combine 5 mL of the hemoglobin solution with the appropriate amount of pepsin (the final concentration should be 2 nM).
 +
## Do the same only with an appropriate amount of pepstatin added. (Each group will use a different concentration of pepstatin. We'll want 2 nM, 200 nM, 20uM, 2mM, and 20mM)
==Data==
==Data==

Revision as of 22:53, 23 September 2013

Biomaterials Design Lab Main project page
Previous entry      Next entry



Objective

To observe the catalytic activity of pepsin (in cleaving peptide bonds in hemoglobin) in the presence and absence of pepstatin. This data will be compared with data we take in a later lab on pepsin-AuNPs.

Description

The experimental details (pepsin cleavage of hemoglobin) are similar to this reference.

We will allow the pepsin cleavage of hemoglobin to proceed for two hours with aliquots taken and analyzed every 1/2 hour. We will also run parallel reactions with different concentrations of pepstatin (pepsin inhibitor) present. We will save our aliquots for analysis tomorrow using SDS-PAGE.

  1. Combine 5 mL of the hemoglobin solution with the appropriate amount of pepsin (the final concentration should be 2 nM).
    1. Do the same only with an appropriate amount of pepstatin added. (Each group will use a different concentration of pepstatin. We'll want 2 nM, 200 nM, 20uM, 2mM, and 20mM)

Data

  • Add data and results here...

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.




Personal tools