User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2013/09/25: Difference between revisions
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# Develop/Stain your gel | # Develop/Stain your gel | ||
## Place gel in Fixative Solution (40% methanol, 10% acetic acid, 50% water) for 30 minutes | ## Place gel in Fixative Solution (40% methanol, 10% acetic acid, 50% water) for 30 minutes | ||
## Place gel in Stain Solution (0. | ## Place gel in Stain Solution (0.025% (w/v) Coomassie Blue, 10% acetic acid, 90% water) for 1 hour | ||
## Place gel in Destain Solution (10% acetic acid, 90% water) for 15 minutes | ## Place gel in Destain Solution (10% acetic acid, 90% water) for 15 minutes | ||
### Repeat this step with fresh destain solution 2 more times | ### Repeat this step with fresh destain solution 2 more times |
Revision as of 07:20, 25 September 2013
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ObjectiveTo run SDS-PAGE to observe amount of hemoglobin remaining after yesterday's digestion. This should be a complimentary measurement to our UV-Vis analysis from yesterday. DescriptionWe will be using a Bio-Rad Mini Protean system with pre-cast Mini Protean TGX gels. The manual for this system can be found here We will be running SDS-PAGE followed by gel development with Coomassie Blue staining. Below is a short description of how we will proceed. Please refer to the manual for more detailed instructions
Data
NotesThis area is for any observations or conclusions that you would like to note.
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