User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2013/09/25: Difference between revisions
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## Load 20uL of your samples into the appropriate lane of your gel | ## Load 20uL of your samples into the appropriate lane of your gel | ||
# Perform electrophoresis | # Perform electrophoresis | ||
## Run for | ## Run for 40 minutes at 200V (I need to make sure our power source can do this) | ||
# Develop/Stain your gel | # Develop/Stain your gel | ||
## Place gel in Fixative Solution (40% methanol, 10% acetic acid, 50% water) for 30 minutes | ## Place gel in Fixative Solution (40% methanol, 10% acetic acid, 50% water) for 30 minutes |
Revision as of 11:16, 23 September 2014
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ObjectiveTo run SDS-PAGE to observe amount of hemoglobin remaining after yesterday's digestion. This should be a complimentary measurement to our UV-Vis analysis from yesterday. DescriptionWe will be using a Bio-Rad Mini Protean system with pre-cast Mini Protean TGX gels. The manual for this system can be found here We will be running SDS-PAGE followed by gel development with Coomassie Blue staining. Below is a short description of how we will proceed. Please refer to the manual for more detailed instructions
Data
NotesThis area is for any observations or conclusions that you would like to note.
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