User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2013/10/16: Difference between revisions
(→Data) |
No edit summary |
||
Line 13: | Line 13: | ||
We are going to test the activity of our HRP-NPs today for the catalytic conversion of luminol | We are going to test the activity of our HRP-NPs today for the catalytic conversion of luminol | ||
==Description== | ==Description==we took the UV-vis of HRP from the previous day after it was diluted, 10% HRP and 90% water. We took UV-vis of Cirtrate from august 28. It was already diluted, 10% citrate and 90% citrate. | ||
<br> A 2.0 ml solution of luminol, hydrogen peroxide and buffer is prepared. Initial concentration of luminol is 1.46 mM. Initial concentration of hydrogen peroxide is 44.29. Final concentration of luminol, as told by Dr. Hartings, should be 0.126 mM, and concentration of hydrogen peroxide is 50 times luminols, 6.3 mM. Accordingly, 0.17 mL of luminol and 0.28 mL of hydrogen peroxide are added to 1.55 mL of the buffer to make the 2ml solution. | |||
<br> another solution of concentration ratio 90 Au:1 BSA is prepared. Concentration of gold is 2.33 mM. Concentration of BSA is 15 uM. 1.3 mL of gold and 2.24 mL of BSA are added to 6.46 ml of water. | |||
==Data== | ==Data== |
Revision as of 11:46, 22 October 2013
Biomaterials Design Lab | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveWe are going to test the activity of our HRP-NPs today for the catalytic conversion of luminol ==Description==we took the UV-vis of HRP from the previous day after it was diluted, 10% HRP and 90% water. We took UV-vis of Cirtrate from august 28. It was already diluted, 10% citrate and 90% citrate.
DataStock Solution
NotesThis area is for any observations or conclusions that you would like to note.
|