User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2013/12/05: Difference between revisions

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==Description==
==Description==
# Add experimental record here. Include what, how, and why...
<u>Stock solutions</u>
# HAuCl<sub>4</sub>
## 24.6 mg
## MW = 393.85 g/mol
## Volume = 25 mL
## 2.5mM
# BSA
## 10.0 mg
## MW = 66776 g/mol
## Volume = 10 mL
## 15.0 uM
# Horseradish peroxidase
## 8.3 mg
## MW = 44000 g/mol
## Volume = 10 mL
## 18.9 uM
# Pepsin
## 6.9 mg
## MW = 34620 g/mol
## Volume = 10 mL
## 19.9 uM
# Myoglobin
## 2.7 mg
## MW = 16951 g/mol
## Volume = 25 mL
## 15.7 uM
# Lysozyme
## 2.5 mg
## MW = 17.1 g/mol
## Volume = 25 mL
## 17.1 uM
# hemoglobin
## 15.2 mg
## MW = 64500 g/mol
## Volume = 10 mL
## 23.6 uM
# Invertase
## 46.7 mg
## MW = 270000 g/mol
## Volume = 10 mL
## 16.3 uM
# Catalase
## 40.8 mg
## MW = 60000 g/mol
## Volume = 10 mL
## 68 uM
 
<u>Samples</u>
 
[[Image:GoldProteinVolumes20131205 2.png | 500px]]


==Data==
==Data==
* Add data and results here...
These pictures were taken the following day.
 
[[Image:BSA AuNPs20131206.jpg | 600px]]
 
[[Image:Myoglobin AuNPs20131206.jpg | 600px]]
 
[[Image:Myoglobin 2AuNPs20131206.jpg | 600px]]
 
[[Image:LysozymeAuNPs20131206.jpg | 600px]]
 
[[Image:PepsinAuNPs20131206.jpg | 600px]]
 
[[Image:HbAuNPs20131206.jpg | 600px]]
 
[[Image:Hb 2AuNPs20131206.jpg | 250px]]
 
[[Image:InvertaseAuNPs20131206.jpg | 600px]]
 
[[Image:CatalaseAuNPs20131206.jpg | 600px]]
 
[[Image:HRPAuNPs20131206.jpg | 600px]]


==Notes==
==Notes==

Revision as of 11:47, 19 March 2014

Biomaterials Design Lab <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Objective

Setting up Protein-AuNP samples for TEM at NIST

Description

Stock solutions

  1. HAuCl4
    1. 24.6 mg
    2. MW = 393.85 g/mol
    3. Volume = 25 mL
    4. 2.5mM
  2. BSA
    1. 10.0 mg
    2. MW = 66776 g/mol
    3. Volume = 10 mL
    4. 15.0 uM
  3. Horseradish peroxidase
    1. 8.3 mg
    2. MW = 44000 g/mol
    3. Volume = 10 mL
    4. 18.9 uM
  4. Pepsin
    1. 6.9 mg
    2. MW = 34620 g/mol
    3. Volume = 10 mL
    4. 19.9 uM
  5. Myoglobin
    1. 2.7 mg
    2. MW = 16951 g/mol
    3. Volume = 25 mL
    4. 15.7 uM
  6. Lysozyme
    1. 2.5 mg
    2. MW = 17.1 g/mol
    3. Volume = 25 mL
    4. 17.1 uM
  7. hemoglobin
    1. 15.2 mg
    2. MW = 64500 g/mol
    3. Volume = 10 mL
    4. 23.6 uM
  8. Invertase
    1. 46.7 mg
    2. MW = 270000 g/mol
    3. Volume = 10 mL
    4. 16.3 uM
  9. Catalase
    1. 40.8 mg
    2. MW = 60000 g/mol
    3. Volume = 10 mL
    4. 68 uM

Samples

Data

These pictures were taken the following day.

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.




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