User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2014/03/11: Difference between revisions
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==Description== | ==Description== | ||
# | I made the following solutions: | ||
# HAuCl<sub>4</sub> | |||
## 24.6mg | |||
## 25.0 mL water | |||
# Hemoglobin | |||
## 15.2mg | |||
## 10.0 mL water | |||
# Catalase | |||
## 40.8mg | |||
## 10.0 mL water | |||
I made HbAuNP samples similar to [[User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/12/05 | this procedure]]. Also, there are two ways to think about the ratio of Au:Hb. Hb is a tetramer. So, the molar mass is either 16125 (for the monomer) or 64500 (for the tetramer). The ratios listed below are for the tetramer/monomer. The ratios as reported in the referenced notebook page are those for the monomer. | |||
# Au:Hb Ratio = 30/7.5 | |||
## 500uL gold | |||
## 1766uL Hb | |||
## 2734uL water | |||
# Au:Hb Ratio = 50/12.5 | |||
## 500uL gold | |||
## 1059uL Hb | |||
## 757uL water | |||
# Au:Hb Ratio = 70/17.5 | |||
## 500uL gold | |||
## 757uL Hb | |||
## 3743uL water | |||
# Au:Catalase Ratio = 90/22.5 | |||
## 500uL gold | |||
## 589uL Hb | |||
## 3911uL water | |||
# Au:Catalase Ratio = 110/27.5 | |||
## 500uL gold | |||
## 482uL Hb | |||
## 4018uL water | |||
# Au:Catalase Ratio = 130/32.5 | |||
## 500uL gold | |||
## 407uL Hb | |||
## 4093uL water | |||
# Au:Catalase Ratio = 150/37.5 | |||
## 500uL gold | |||
## 353uL Hb | |||
## 4147uL water | |||
# Au:Catalase Ratio = 170/42.5 | |||
## 500uL gold | |||
## 312uL Hb | |||
## 4188uL water | |||
# Au:Catalase Ratio = 190/47.5 | |||
## 500uL gold | |||
## 279uL Hb | |||
## 4221uL water | |||
# Au:Catalase Ratio = 210/52.5 | |||
## 500uL gold | |||
## 252uL Hb | |||
## 4248uL water | |||
In order to get a more detailed view of the Catalase trends I made the following solutions: | |||
# Au:Catalase Ratio = 7 | |||
## 500uL gold | |||
## 2556uL catalase | |||
## 1944uL water | |||
# Au:Catalase Ratio = 17 | |||
## 500uL gold | |||
## 1096uL catalase | |||
## 3404uL water | |||
# Au:Catalase Ratio = 26 | |||
## 500uL gold | |||
## 697uL catalase | |||
## 3803uL water | |||
# Au:Catalase Ratio = 36 | |||
## 500uL gold | |||
## 511uL catalase | |||
## 3989uL water | |||
# Au:Catalase Ratio = 46 | |||
## 500uL gold | |||
## 404uL catalase | |||
## 4096uL water | |||
# Au:Catalase Ratio = 55 | |||
## 500uL gold | |||
## 333uL catalase | |||
## 4167uL water | |||
# Au:Catalase Ratio = 65 | |||
## 500uL gold | |||
## 284uL catalase | |||
## 4216uL water | |||
# Au:Catalase Ratio = 74 | |||
## 500uL gold | |||
## 247uL catalase | |||
## 4253uL water | |||
# Au:Catalase Ratio = 84 | |||
## 500uL gold | |||
## 219uL catalase | |||
## 4281uL water | |||
# Au:Catalase Ratio = 93 | |||
## 500uL gold | |||
## 197uL catalase | |||
## 4303uL water | |||
I also took UVVis of the gold, Hb, and catalase stock solutions. | |||
For each, I measured the spectrum for a 10X dilution of each stock solution. | |||
==Data== | ==Data== | ||
Here is a picture of the HbAuNPs (left - 30/7.5, right - 210/52.5) | |||
[[Image:HbAuNPpic20140311.jpg | 400px]] | |||
Here is a picture of the CatalaseAuNPs (left - 390, right - 30) | |||
[[Image:CatalaseAuNPpic20140311.jpg | 400px]] | |||
==Notes== | ==Notes== |
Revision as of 12:57, 19 March 2014
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ObjectiveMaking new catalase and Hb AuNP samples, as described here for AA and UVVis analysis. DescriptionI made the following solutions:
I made HbAuNP samples similar to this procedure. Also, there are two ways to think about the ratio of Au:Hb. Hb is a tetramer. So, the molar mass is either 16125 (for the monomer) or 64500 (for the tetramer). The ratios listed below are for the tetramer/monomer. The ratios as reported in the referenced notebook page are those for the monomer.
In order to get a more detailed view of the Catalase trends I made the following solutions:
I also took UVVis of the gold, Hb, and catalase stock solutions. For each, I measured the spectrum for a 10X dilution of each stock solution. DataHere is a picture of the HbAuNPs (left - 30/7.5, right - 210/52.5) Here is a picture of the CatalaseAuNPs (left - 390, right - 30) NotesThis area is for any observations or conclusions that you would like to note.
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