User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2015/09/02

Biomaterials Design Lab

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Objective Prepping stock solutions for use in making AuNP fibers with lysozyme for other researchers to use. Description Target Concentrations Based on the total reaction volume that the class will be using and the final concentrations that will be in each reaction volume, I have determined that the following HAuCl4·H2O and Lysozyme masses are appropriate for today's stock solutions. Stock solutions HAuCl4·H2O 2.5 mM MW = 339.79 g/mol Volume = 50 mL 42.4 mg Lysozyme 20 μM MW = 14307 g/mol Volume = 100 mL 28.6 mg Actual Concentrations The actual masses are different from the target masses. The concentrations shown below reflect the actual concentrations made for the stock solutions. I used a 50 mL volumetric flask to make the gold stock solution and a 100 mL volumetric flask to make the protein solution. In each case I used HPLC-grade water. Stock solutions HAuCl4·H2O 50.32 mg MW = 339.79 g/mol Volume = 50 mL 2.96 mM Lysozyme 30.65 mg MW = 14307 g/mol Volume = 100 mL 21.4 μM Directions for making samples Note: The final concentration of gold should be 0.25 mM and the final concentration of lysozyme should be 5.556 μM. This gives a Au:lysozyme ratio of 45, which should yield fibers. 1 mL samples Volume of gold stock: 84.4 μL Volume of protein stock: 259 μL Volume of water: 656 μL 5 mL samples Volume of gold stock: 422 μL Volume of protein stock: 1,297 μL Volume of water: 3,281 μL I also made 1mL fiber samples stock concentrations I used an old gold sample (2.96 mM) Lysozyme 4.60 mg lysozyme 5 mL volumetric 64.3 uM reaction samples 86.4 uL protein 84.5 uL gold 829 uL water I placed all of the samples in a rack and covered with aluminum foil and placed in the oven. Data Add data and results here... Notes This area is for any observations or conclusions that you would like to note. Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.