User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2015/09/16: Difference between revisions
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==Objective== | ==Objective== | ||
# Prep 1 mL fiber samples for analysis | |||
## Centrifuge your samples and remove the supernatant. | |||
## Leave the tubes open so that rest of the water can evaporate | |||
# If your samples from yesterday didn't work, remake them | |||
# Make 500 mL of 10 mM Tris buffer, pH 9 | |||
# Make 500 mL of 10 mM phosphate buffer, pH 7 | |||
## Use the meter at your bench to check the pH and conductivity of you samples | |||
## You will need to calibrate for each measurement | |||
## I will check your buffers when you are finished | |||
## You will have to make each buffer until your readings (pH and conductivity) come out to reasonable numbers | |||
### The phosphate buffer should be made using only sodium phosphate (dibasic) and sodium phosphate (monobasic) this will set the pH AND conductivity of your sample | |||
### The conductivity of your Tris buffer will change a bit and will reflect a higher ion concentration than your 10 mM Tris would suggest. | |||
==Description== | ==Description== |
Revision as of 17:23, 15 September 2015
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Objective
Description
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NotesThis area is for any observations or conclusions that you would like to note.
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