User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2016/08/29: Difference between revisions

Revision as of 09:58, 29 August 2016

Biomaterials Design Lab

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Objective

Calculations for stock solutions to be made tomorrow.

Description

stock solutions

1 L of 0.001 M HCl
1. Concentrated HCl should be: 11.65 M
2. M₁V₁ = M₂V₂
3. (11.65 M)(V₁) = (0.001M)(1L)
4. V₁ = 85.8 μL
1 L of 0.001 M HNO₃
1. Concentrated HNO₃ should be: 15.8 M
2. M₁V₁ = M₂V₂
3. (15.8 M)(V₁) = (0.001M)(1L)
4. V₁ = 63.2 μL
1 L of 1 M NaOH
1. MW of NaOH = 39.997 g/mol
2. M = (m/MW)/V
3. 1 M = (m/39.997 g/mol)/1L
4. m = 39.997 g
1 L of 0.001 M NaOH
1. Use 1M stock solution
2. M₁V₁ = M₂V₂
3. (1 M)(V₁) = (0.001M)(1L)
4. V₁ = 1 mL
10 mL of 12.5 mM Fructose
1. MW = 180.16 g/mol
2. 0.0125 M = (m/180.16 g/mol)/(0.010 L)
3. m = 22.5 mg
10 mL of 2.5 mM AuCl₃
1. MW = 303.33 g/mol
2. 0.0025 M = (m/303.33 g/mol)/(0.010 L)
3. m = 7.58 mg

sample solutions

0.25 mM AuCl₃, 0 mM fructose, pH 4 ([H⁺] = 1x10^-4 M), 5 mL total
1. Volume of AuCl₃ stock
  1. M₁V₁ = M₂V₂
  2. (0.0025 M)V₁ = (0.00025 M)(5 mL)
  3. V_{AuCl₃ stock} = 0.5 mL
2. Volume of fructose stock
  1. V_{fructose stock} = 0 mL
3. Volume of HCl stock
  1. M₁V₁ = M₂V₂
  2. (1x10^-3 M)V₁ = (1x10^-4 M)(5 mL)
  3. V_{HCl stock} = 0.5 mL
4. Volume of water
  1. V_water = 5 mL - V_{AuCl₃ stock} - V_{fructose stock} - V_{HCl stock}
  2. V_water = 4 mL
0.25 mM AuCl₃, 0 mM fructose, pH 5 ([H⁺] = 1x10^-5 M), 5 mL total
1. Volume of AuCl₃ stock
  1. M₁V₁ = M₂V₂
  2. (0.0025 M)V₁ = (0.00025 M)(5 mL)
  3. V_{AuCl₃ stock} = 0.5 mL
2. Volume of fructose stock
  1. V_{fructose stock} = 0 mL
3. Volume of HCl stock
  1. M₁V₁ = M₂V₂
  2. (1x10^-3 M)V₁ = (1x10^-5 M)(5 mL)
  3. V_{HCl stock} = 0.05 mL
4. Volume of water
  1. V_water = 5 mL - V_{AuCl₃ stock} - V_{fructose stock} - V_{HCl stock}
  2. V_water = 4.45 mL
0.25 mM AuCl₃, 0 mM fructose, pH 6 ([H⁺] = 1x10^-6 M), 5 mL total
1. Volume of AuCl₃ stock
  1. M₁V₁ = M₂V₂
  2. (0.0025 M)V₁ = (0.00025 M)(5 mL)
  3. V_{AuCl₃ stock} = 0.5 mL
2. Volume of fructose stock
  1. V_{fructose stock} = 0 mL
3. Volume of HCl stock
  1. M₁V₁ = M₂V₂
  2. (1x10^-3 M)V₁ = (1x10^-6 M)(5 mL)
  3. V_{HCl stock} = 0.005 mL
4. Volume of water
  1. V_water = 5 mL - V_{AuCl₃ stock} - V_{fructose stock} - V_{HCl stock}
  2. V_water = 4.495 mL
0.25 mM AuCl₃, 0 mM fructose, pH 7 ([H⁺] = 1x10^-7 M), 5 mL total
1. Volume of AuCl₃ stock
  1. M₁V₁ = M₂V₂
  2. (0.0025 M)V₁ = (0.00025 M)(5 mL)
  3. V_{AuCl₃ stock} = 0.5 mL
2. Volume of fructose stock
  1. V_{fructose stock} = 0 mL
3. Volume of HCl stock
  1. V_{HCl stock} = 0 mL
4. Volume of water
  1. V_water = 5 mL - V_{AuCl₃ stock} - V_{fructose stock} - V_{HCl stock}
  2. V_water = 4.5 mL
0.25 mM AuCl₃, 0 mM fructose, pH 8 ([H⁺] = 1x10^-8 M), 5 mL total
1. Volume of AuCl₃ stock
  1. M₁V₁ = M₂V₂
  2. (0.0025 M)V₁ = (0.00025 M)(5 mL)
  3. V_{AuCl₃ stock} = 0.5 mL
2. Volume of fructose stock
  1. V_{fructose stock} = 0 mL
3. Volume of 0.001 M NaOH stock
  1. M₁V₁ = M₂V₂
  2. (1x10^-3 M)V₁ = (1x10^-6 M)(5 mL)
  3. V_{NaOH stock} = 0.005 mL
4. Volume of water
  1. V_water = 5 mL - V_{AuCl₃ stock} - V_{fructose stock} - V_{NaOH stock}
  2. V_water = 4.495 mL
0.25 mM AuCl₃, 0 mM fructose, pH 9 ([H⁺] = 1x10^-9 M), 5 mL total
1. Volume of AuCl₃ stock
  1. M₁V₁ = M₂V₂
  2. (0.0025 M)V₁ = (0.00025 M)(5 mL)
  3. V_{AuCl₃ stock} = 0.5 mL
2. Volume of fructose stock
  1. V_{fructose stock} = 0 mL
3. Volume of 0.001 M NaOH stock
  1. M₁V₁ = M₂V₂
  2. (1x10^-3 M)V₁ = (1x10^-5 M)(5 mL)
  3. V_{NaOH stock} = 0.05 mL
4. Volume of water
  1. V_water = 5 mL - V_{AuCl₃ stock} - V_{fructose stock} - V_{NaOH stock}
  2. V_water = 4.45 mL
0.25 mM AuCl₃, 0 mM fructose, pH 10 ([H⁺] = 1x10^-10 M), 5 mL total
1. Volume of AuCl₃ stock
  1. M₁V₁ = M₂V₂
  2. (0.0025 M)V₁ = (0.00025 M)(5 mL)
  3. V_{AuCl₃ stock} = 0.5 mL
2. Volume of fructose stock
  1. V_{fructose stock} = 0 mL
3. Volume of 0.001 M NaOH stock
  1. M₁V₁ = M₂V₂
  2. (1x10^-3 M)V₁ = (1x10^-4 M)(5 mL)
  3. V_{NaOH stock} = 0.5 mL
4. Volume of water
  1. V_water = 5 mL - V_{AuCl₃ stock} - V_{fructose stock} - V_{NaOH stock}
  2. V_water = 4 mL
0.25 mM AuCl₃, 0 mM fructose, pH 11 ([H⁺] = 1x10^-11 M), 5 mL total
1. Volume of AuCl₃ stock
  1. M₁V₁ = M₂V₂
  2. (0.0025 M)V₁ = (0.00025 M)(5 mL)
  3. V_{AuCl₃ stock} = 0.5 mL
2. Volume of fructose stock
  1. V_{fructose stock} = 0 mL
3. Volume of 1 M NaOH stock
  1. M₁V₁ = M₂V₂
  2. (1 M)V₁ = (1x10^-3 M)(5 mL)
  3. V_{NaOH stock} = 0.005 mL
4. Volume of water
  1. V_water = 5 mL - V_{AuCl₃ stock} - V_{fructose stock} - V_{NaOH stock}
  2. V_water = 4.495 mL
0.25 mM AuCl₃, 0 mM fructose, pH 12 ([H⁺] = 1x10^-12 M), 5 mL total
1. Volume of AuCl₃ stock
  1. M₁V₁ = M₂V₂
  2. (0.0025 M)V₁ = (0.00025 M)(5 mL)
  3. V_{AuCl₃ stock} = 0.5 mL
2. Volume of fructose stock
  1. V_{fructose stock} = 0 mL
3. Volume of 1 M NaOH stock
  1. M₁V₁ = M₂V₂
  2. (1 M)V₁ = (1x10^-2 M)(5 mL)
  3. V_{NaOH stock} = 0.05 mL
4. Volume of water
  1. V_water = 5 mL - V_{AuCl₃ stock} - V_{fructose stock} - V_{NaOH stock}
  2. V_water = 4.45 mL

Data

Add data and results here...

Notes

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Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.

@@ Line 14: / Line 14: @@
 ==Description==
-# Add experimental record here. Include what, how, and why...
+<u>stock solutions</u>
+# 1 L of 0.001 M HCl
+## Concentrated HCl should be: 11.65 M
+## M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub>
+## (11.65 M)(V<sub>1</sub>) = (0.001M)(1L)
+## V<sub>1</sub> = 85.8 μL
+# 1 L of 0.001 M HNO<sub>3</sub>
+## Concentrated HNO<sub>3</sub> should be: 15.8 M
+## M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub>
+## (15.8 M)(V<sub>1</sub>) = (0.001M)(1L)
+## V<sub>1</sub> = 63.2 μL
+# 1 L of 1 M NaOH
+## MW of NaOH = 39.997 g/mol
+## M = (m/MW)/V
+## 1 M = (m/39.997 g/mol)/1L
+## m = 39.997 g
+# 1 L of 0.001 M NaOH
+## Use 1M stock solution
+## M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub>
+## (1 M)(V<sub>1</sub>) = (0.001M)(1L)
+## V<sub>1</sub> = 1 mL
+# 10 mL of 12.5 mM Fructose
+## MW = 180.16 g/mol
+## 0.0125 M = (m/180.16 g/mol)/(0.010 L)
+## m = 22.5 mg
+# 10 mL of 2.5 mM AuCl<sub>3</sub>
+## MW = 303.33 g/mol
+## 0.0025 M = (m/303.33 g/mol)/(0.010 L)
+## m = 7.58 mg
+<u> sample solutions </u>
+# 0.25 mM AuCl<sub>3</sub>, 0 mM fructose, pH 4 ([H<sup>+</sup>] = 1x10<sup>-4</sup> M), 5 mL total
+## Volume of AuCl<sub>3</sub> stock
+### M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub>
+### (0.0025 M)V<sub>1</sub> = (0.00025 M)(5 mL)
+### V<sub>AuCl<sub>3</sub> stock</sub> = 0.5 mL
+## Volume of fructose stock
+### V<sub>fructose stock</sub> = 0 mL
+## Volume of HCl stock
+### M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub>
+### (1x10<sup>-3</sup> M)V<sub>1</sub> = (1x10<sup>-4</sup> M)(5 mL)
+### V<sub>HCl stock</sub> = 0.5 mL
+## Volume of water
+### V<sub>water</sub> = 5 mL - V<sub>AuCl<sub>3</sub> stock</sub> - V<sub>fructose stock</sub> - V<sub>HCl stock</sub>
+### V<sub>water</sub> = 4 mL
+# 0.25 mM AuCl<sub>3</sub>, 0 mM fructose, pH 5 ([H<sup>+</sup>] = 1x10<sup>-5</sup> M), 5 mL total
+## Volume of AuCl<sub>3</sub> stock
+### M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub>
+### (0.0025 M)V<sub>1</sub> = (0.00025 M)(5 mL)
+### V<sub>AuCl<sub>3</sub> stock</sub> = 0.5 mL
+## Volume of fructose stock
+### V<sub>fructose stock</sub> = 0 mL
+## Volume of HCl stock
+### M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub>
+### (1x10<sup>-3</sup> M)V<sub>1</sub> = (1x10<sup>-5</sup> M)(5 mL)
+### V<sub>HCl stock</sub> = 0.05 mL
+## Volume of water
+### V<sub>water</sub> = 5 mL - V<sub>AuCl<sub>3</sub> stock</sub> - V<sub>fructose stock</sub> - V<sub>HCl stock</sub>
+### V<sub>water</sub> = 4.45 mL
+# 0.25 mM AuCl<sub>3</sub>, 0 mM fructose, pH 6 ([H<sup>+</sup>] = 1x10<sup>-6</sup> M), 5 mL total
+## Volume of AuCl<sub>3</sub> stock
+### M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub>
+### (0.0025 M)V<sub>1</sub> = (0.00025 M)(5 mL)
+### V<sub>AuCl<sub>3</sub> stock</sub> = 0.5 mL
+## Volume of fructose stock
+### V<sub>fructose stock</sub> = 0 mL
+## Volume of HCl stock
+### M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub>
+### (1x10<sup>-3</sup> M)V<sub>1</sub> = (1x10<sup>-6</sup> M)(5 mL)
+### V<sub>HCl stock</sub> = 0.005 mL
+## Volume of water
+### V<sub>water</sub> = 5 mL - V<sub>AuCl<sub>3</sub> stock</sub> - V<sub>fructose stock</sub> - V<sub>HCl stock</sub>
+### V<sub>water</sub> = 4.495 mL
+# 0.25 mM AuCl<sub>3</sub>, 0 mM fructose, pH 7 ([H<sup>+</sup>] = 1x10<sup>-7</sup> M), 5 mL total
+## Volume of AuCl<sub>3</sub> stock
+### M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub>
+### (0.0025 M)V<sub>1</sub> = (0.00025 M)(5 mL)
+### V<sub>AuCl<sub>3</sub> stock</sub> = 0.5 mL
+## Volume of fructose stock
+### V<sub>fructose stock</sub> = 0 mL
+## Volume of HCl stock
+### V<sub>HCl stock</sub> = 0 mL
+## Volume of water
+### V<sub>water</sub> = 5 mL - V<sub>AuCl<sub>3</sub> stock</sub> - V<sub>fructose stock</sub> - V<sub>HCl stock</sub>
+### V<sub>water</sub> = 4.5 mL
+# 0.25 mM AuCl<sub>3</sub>, 0 mM fructose, pH 8 ([H<sup>+</sup>] = 1x10<sup>-8</sup> M), 5 mL total
+## Volume of AuCl<sub>3</sub> stock
+### M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub>
+### (0.0025 M)V<sub>1</sub> = (0.00025 M)(5 mL)
+### V<sub>AuCl<sub>3</sub> stock</sub> = 0.5 mL
+## Volume of fructose stock
+### V<sub>fructose stock</sub> = 0 mL
+## Volume of 0.001 M NaOH stock
+### M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub>
+### (1x10<sup>-3</sup> M)V<sub>1</sub> = (1x10<sup>-6</sup> M)(5 mL)
+### V<sub>NaOH stock</sub> = 0.005 mL
+## Volume of water
+### V<sub>water</sub> = 5 mL - V<sub>AuCl<sub>3</sub> stock</sub> - V<sub>fructose stock</sub> - V<sub>NaOH stock</sub>
+### V<sub>water</sub> = 4.495 mL
+# 0.25 mM AuCl<sub>3</sub>, 0 mM fructose, pH 9 ([H<sup>+</sup>] = 1x10<sup>-9</sup> M), 5 mL total
+## Volume of AuCl<sub>3</sub> stock
+### M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub>
+### (0.0025 M)V<sub>1</sub> = (0.00025 M)(5 mL)
+### V<sub>AuCl<sub>3</sub> stock</sub> = 0.5 mL
+## Volume of fructose stock
+### V<sub>fructose stock</sub> = 0 mL
+## Volume of 0.001 M NaOH stock
+### M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub>
+### (1x10<sup>-3</sup> M)V<sub>1</sub> = (1x10<sup>-5</sup> M)(5 mL)
+### V<sub>NaOH stock</sub> = 0.05 mL
+## Volume of water
+### V<sub>water</sub> = 5 mL - V<sub>AuCl<sub>3</sub> stock</sub> - V<sub>fructose stock</sub> - V<sub>NaOH stock</sub>
+### V<sub>water</sub> = 4.45 mL
+# 0.25 mM AuCl<sub>3</sub>, 0 mM fructose, pH 10 ([H<sup>+</sup>] = 1x10<sup>-10</sup> M), 5 mL total
+## Volume of AuCl<sub>3</sub> stock
+### M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub>
+### (0.0025 M)V<sub>1</sub> = (0.00025 M)(5 mL)
+### V<sub>AuCl<sub>3</sub> stock</sub> = 0.5 mL
+## Volume of fructose stock
+### V<sub>fructose stock</sub> = 0 mL
+## Volume of 0.001 M NaOH stock
+### M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub>
+### (1x10<sup>-3</sup> M)V<sub>1</sub> = (1x10<sup>-4</sup> M)(5 mL)
+### V<sub>NaOH stock</sub> = 0.5 mL
+## Volume of water
+### V<sub>water</sub> = 5 mL - V<sub>AuCl<sub>3</sub> stock</sub> - V<sub>fructose stock</sub> - V<sub>NaOH stock</sub>
+### V<sub>water</sub> = 4 mL
+# 0.25 mM AuCl<sub>3</sub>, 0 mM fructose, pH 11 ([H<sup>+</sup>] = 1x10<sup>-11</sup> M), 5 mL total
+## Volume of AuCl<sub>3</sub> stock
+### M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub>
+### (0.0025 M)V<sub>1</sub> = (0.00025 M)(5 mL)
+### V<sub>AuCl<sub>3</sub> stock</sub> = 0.5 mL
+## Volume of fructose stock
+### V<sub>fructose stock</sub> = 0 mL
+## Volume of 1 M NaOH stock
+### M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub>
+### (1 M)V<sub>1</sub> = (1x10<sup>-3</sup> M)(5 mL)
+### V<sub>NaOH stock</sub> = 0.005 mL
+## Volume of water
+### V<sub>water</sub> = 5 mL - V<sub>AuCl<sub>3</sub> stock</sub> - V<sub>fructose stock</sub> - V<sub>NaOH stock</sub>
+### V<sub>water</sub> = 4.495 mL
+# 0.25 mM AuCl<sub>3</sub>, 0 mM fructose, pH 12 ([H<sup>+</sup>] = 1x10<sup>-12</sup> M), 5 mL total
+## Volume of AuCl<sub>3</sub> stock
+### M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub>
+### (0.0025 M)V<sub>1</sub> = (0.00025 M)(5 mL)
+### V<sub>AuCl<sub>3</sub> stock</sub> = 0.5 mL
+## Volume of fructose stock
+### V<sub>fructose stock</sub> = 0 mL
+## Volume of 1 M NaOH stock
+### M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub>
+### (1 M)V<sub>1</sub> = (1x10<sup>-2</sup> M)(5 mL)
+### V<sub>NaOH stock</sub> = 0.05 mL
+## Volume of water
+### V<sub>water</sub> = 5 mL - V<sub>AuCl<sub>3</sub> stock</sub> - V<sub>fructose stock</sub> - V<sub>NaOH stock</sub>
+### V<sub>water</sub> = 4.45 mL
 ==Data==

User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2016/08/29: Difference between revisions

Revision as of 09:58, 29 August 2016

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