Objective
Calculations for stock solutions to be made tomorrow.
Description
stock solutions
- 1 L of 0.001 M HCl
- Concentrated HCl should be: 11.65 M
- M1V1 = M2V2
- (11.65 M)(V1) = (0.001M)(1L)
- V1 = 85.8 μL
- 1 L of 0.001 M HNO3
- Concentrated HNO3 should be: 15.8 M
- M1V1 = M2V2
- (15.8 M)(V1) = (0.001M)(1L)
- V1 = 63.2 μL
- 1 L of 1 M NaOH
- MW of NaOH = 39.997 g/mol
- M = (m/MW)/V
- 1 M = (m/39.997 g/mol)/1L
- m = 39.997 g
- 1 L of 0.001 M NaOH
- Use 1M stock solution
- M1V1 = M2V2
- (1 M)(V1) = (0.001M)(1L)
- V1 = 1 mL
- 10 mL of 12.5 mM Fructose
- MW = 180.16 g/mol
- 0.0125 M = (m/180.16 g/mol)/(0.010 L)
- m = 22.5 mg
- 10 mL of 2.5 mM AuCl3
- MW = 303.33 g/mol
- 0.0025 M = (m/303.33 g/mol)/(0.010 L)
- m = 7.58 mg
- Protein (BSA)
- MW = 66776 g/mol
- 30 μM = (m/66776 g/mol)/(0.010 L)
- m = 20.0 mg
sample solutions
- 0.25 mM AuCl3, 0 mM fructose, pH 4 ([H+] = 1x10-4 M), 5 mL total
- Volume of AuCl3 stock
- M1V1 = M2V2
- (0.0025 M)V1 = (0.00025 M)(5 mL)
- VAuCl3 stock = 0.5 mL
- Volume of fructose stock
- Vfructose stock = 0 mL
- Volume of HCl stock
- M1V1 = M2V2
- (1x10-3 M)V1 = (1x10-4 M)(5 mL)
- VHCl stock = 0.5 mL
- Volume of Protein stock
- M1V1 = M2V2
- (30 μM)VProtein Stock = (3.1 μM))(5 mL)
- VProtein Stock = 0.517 mL
- Volume of water
- Vwater = 5 mL - VAuCl3 stock - Vfructose stock - VHCl stock - VProtein Stock
- Vwater = 3.483 mL
- 0.25 mM AuCl3, 0 mM fructose, pH 5 ([H+] = 1x10-5 M), 5 mL total
- Volume of AuCl3 stock
- M1V1 = M2V2
- (0.0025 M)V1 = (0.00025 M)(5 mL)
- VAuCl3 stock = 0.5 mL
- Volume of fructose stock
- Vfructose stock = 0 mL
- Volume of HCl stock
- M1V1 = M2V2
- (1x10-3 M)V1 = (1x10-5 M)(5 mL)
- VHCl stock = 0.05 mL
- Volume of Protein stock
- M1V1 = M2V2
- (30 μM)VProtein Stock = (3.1 μM))(5 mL)
- VProtein Stock = 0.517 mL
- Volume of water
- Vwater = 5 mL - VAuCl3 stock - Vfructose stock - VHCl stock - VProtein Stock
- Vwater = 3.933 mL
- 0.25 mM AuCl3, 0 mM fructose, pH 6 ([H+] = 1x10-6 M), 5 mL total
- Volume of AuCl3 stock
- M1V1 = M2V2
- (0.0025 M)V1 = (0.00025 M)(5 mL)
- VAuCl3 stock = 0.5 mL
- Volume of fructose stock
- Vfructose stock = 0 mL
- Volume of HCl stock
- M1V1 = M2V2
- (1x10-3 M)V1 = (1x10-6 M)(5 mL)
- VHCl stock = 0.005 mL
- Volume of Protein stock
- M1V1 = M2V2
- (30 μM)VProtein Stock = (3.1 μM))(5 mL)
- VProtein Stock = 0.517 mL
- Volume of water
- Vwater = 5 mL - VAuCl3 stock - Vfructose stock - VHCl stock - VProtein Stock
- Vwater = 3.978 mL
- 0.25 mM AuCl3, 0 mM fructose, pH 7 ([H+] = 1x10-7 M), 5 mL total
- Volume of AuCl3 stock
- M1V1 = M2V2
- (0.0025 M)V1 = (0.00025 M)(5 mL)
- VAuCl3 stock = 0.5 mL
- Volume of fructose stock
- Vfructose stock = 0 mL
- Volume of HCl stock
- VHCl stock = 0 mL
- Volume of Protein stock
- M1V1 = M2V2
- (30 μM)VProtein Stock = (3.1 μM))(5 mL)
- VProtein Stock = 0.517 mL
- Volume of water
- Vwater = 5 mL - VAuCl3 stock - Vfructose stock - VHCl stock - VProtein Stock
- Vwater = 3.983 mL
- 0.25 mM AuCl3, 0 mM fructose, pH 8 ([H+] = 1x10-8 M), 5 mL total
- Volume of AuCl3 stock
- M1V1 = M2V2
- (0.0025 M)V1 = (0.00025 M)(5 mL)
- VAuCl3 stock = 0.5 mL
- Volume of fructose stock
- Vfructose stock = 0 mL
- Volume of 0.001 M NaOH stock
- M1V1 = M2V2
- (1x10-3 M)V1 = (1x10-6 M)(5 mL)
- VNaOH stock = 0.005 mL
- Volume of Protein stock
- M1V1 = M2V2
- (30 μM)VProtein Stock = (3.1 μM))(5 mL)
- VProtein Stock = 0.517 mL
- Volume of water
- Vwater = 5 mL - VAuCl3 stock - Vfructose stock - VNaOH stock - VProtein Stock
- Vwater = 3.978 mL
- 0.25 mM AuCl3, 0 mM fructose, pH 9 ([H+] = 1x10-9 M), 5 mL total
- Volume of AuCl3 stock
- M1V1 = M2V2
- (0.0025 M)V1 = (0.00025 M)(5 mL)
- VAuCl3 stock = 0.5 mL
- Volume of fructose stock
- Vfructose stock = 0 mL
- Volume of 0.001 M NaOH stock
- M1V1 = M2V2
- (1x10-3 M)V1 = (1x10-5 M)(5 mL)
- VNaOH stock = 0.05 mL
- Volume of Protein stock
- M1V1 = M2V2
- (30 μM)VProtein Stock = (3.1 μM))(5 mL)
- VProtein Stock = 0.517 mL
- Volume of water
- Vwater = 5 mL - VAuCl3 stock - Vfructose stock - VNaOH stock - VProtein Stock
- Vwater = 3.933 mL
- 0.25 mM AuCl3, 0 mM fructose, pH 10 ([H+] = 1x10-10 M), 5 mL total
- Volume of AuCl3 stock
- M1V1 = M2V2
- (0.0025 M)V1 = (0.00025 M)(5 mL)
- VAuCl3 stock = 0.5 mL
- Volume of fructose stock
- Vfructose stock = 0 mL
- Volume of 0.001 M NaOH stock
- M1V1 = M2V2
- (1x10-3 M)V1 = (1x10-4 M)(5 mL)
- VNaOH stock = 0.5 mL
- Volume of Protein stock
- M1V1 = M2V2
- (30 μM)VProtein Stock = (3.1 μM))(5 mL)
- VProtein Stock = 0.517 mL
- Volume of water
- Vwater = 5 mL - VAuCl3 stock - Vfructose stock - VNaOH stock - VProtein Stock
- Vwater = 3.483 mL
- 0.25 mM AuCl3, 0 mM fructose, pH 11 ([H+] = 1x10-11 M), 5 mL total
- Volume of AuCl3 stock
- M1V1 = M2V2
- (0.0025 M)V1 = (0.00025 M)(5 mL)
- VAuCl3 stock = 0.5 mL
- Volume of fructose stock
- Vfructose stock = 0 mL
- Volume of 1 M NaOH stock
- M1V1 = M2V2
- (1 M)V1 = (1x10-3 M)(5 mL)
- VNaOH stock = 0.005 mL
- Volume of Protein stock
- M1V1 = M2V2
- (30 μM)VProtein Stock = (3.1 μM))(5 mL)
- VProtein Stock = 0.517 mL
- Volume of water
- Vwater = 5 mL - VAuCl3 stock - Vfructose stock - VNaOH stock - VProtein Stock
- Vwater = 3.978 mL
- 0.25 mM AuCl3, 0 mM fructose, pH 12 ([H+] = 1x10-12 M), 5 mL total
- Volume of AuCl3 stock
- M1V1 = M2V2
- (0.0025 M)V1 = (0.00025 M)(5 mL)
- VAuCl3 stock = 0.5 mL
- Volume of fructose stock
- Vfructose stock = 0 mL
- Volume of 1 M NaOH stock
- M1V1 = M2V2
- (1 M)V1 = (1x10-2 M)(5 mL)
- VNaOH stock = 0.05 mL
- Volume of Protein stock
- M1V1 = M2V2
- (30 μM)VProtein Stock = (3.1 μM))(5 mL)
- VProtein Stock = 0.517 mL
- Volume of water
- Vwater = 5 mL - VAuCl3 stock - Vfructose stock - VNaOH stock - VProtein Stock
- Vwater = 3.933 mL
Data
- Add data and results here...
Notes
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