User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2016/08/31: Difference between revisions
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==Data== | ==Data== | ||
<u> Stock solutions </u> | |||
# Lysozyme | |||
## 8.88 mg of lysozyme with water in 10.0 mL volumetric flask | |||
## MW = 14307 g/mol | |||
## concentration = 62.1 μM | |||
# Myoglobin | |||
## 13.71 mg of myoglobin with water in 10.0 mL volumetric flask | |||
## MW = 16951 g/mol | |||
## concentration = 80.9 μM | |||
<u>Instructions for making samples for kinetics experiments</u> | |||
# pH 4 | |||
## Lysozyme: 0.25mM Au, 12.5μM Lysozyme, 3mL total | |||
### 225 μL AuCl<sub>3</sub> | |||
### 300 μL 1M HCl | |||
### 604 μL Lysozyme | |||
### 1871 μL Water | |||
## Myoglobin: 0.25mM Au, 12.5μM Myoglobin, 3mL total | |||
### 225 μL AuCl<sub>3</sub> | |||
### 300 μL 1M HCl | |||
### 464 μL Myoglobin | |||
### 1871 μL Water | |||
==Notes== | ==Notes== |
Revision as of 08:35, 31 August 2016
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ObjectiveSamples for group A Make samples using the stock solutions I prepped yesterday.
Make stock solutions of lysozyme and myoglobin for experiments DescriptionSamples for group A Solutions were added to glass test tubes, covered with aluminum foil, and placed in an oven (set to 80°C) for 4 hours. DataStock solutions
NotesThis area is for any observations or conclusions that you would like to note.
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