User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2016/08/31: Difference between revisions

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[[Image:20160831 mrh BSAAuNP.JPG| 400px]]
[[Image:20160831 mrh BSAAuNP.JPG| 400px]]
pH 4 through 12 (left to right), 0.25 mM Au, 3.1 μM BSA, 0.0625 mM fructose
[[Image:20160831 mph BSAFructoseAuNP.JPG|400px]]


==Notes==
==Notes==

Revision as of 11:19, 31 August 2016

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Objective

Samples for group A

Make samples using the stock solutions I prepped yesterday.


Stock solutions for groups B and C experiments

Make stock solutions of lysozyme and myoglobin for experiments

Description

Samples for group A

Solutions were added to glass test tubes, covered with aluminum foil, and placed in an oven (set to 80°C) for 4 hours.

Data

Stock solutions

  1. Lysozyme
    1. 8.88 mg of lysozyme with water in 10.0 mL volumetric flask
    2. MW = 14307 g/mol
    3. concentration = 62.1 μM
  2. Myoglobin
    1. 13.71 mg of myoglobin with water in 10.0 mL volumetric flask
    2. MW = 16951 g/mol
    3. concentration = 80.9 μM


Instructions for making samples for kinetics experiments

  1. pH 4
    1. Lysozyme: 0.25mM Au, 12.5μM Lysozyme, 3mL total
      1. 225 μL AuCl3
      2. 300 μL 1M HCl
      3. 604 μL Lysozyme
      4. 1871 μL Water
    2. Myoglobin: 0.25mM Au, 12.5μM Myoglobin, 3mL total
      1. 225 μL AuCl3
      2. 300 μL 1M HCl
      3. 464 μL Myoglobin
      4. 1871 μL Water

AuNP synthesis results

pH 4 through 12 (left to right), 0.25 mM Au, 3.1 μM BSA, 0 mM fructose

pH 4 through 12 (left to right), 0.25 mM Au, 3.1 μM BSA, 0.0625 mM fructose

File:20160831 mph BSAFructoseAuNP.JPG

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.




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