User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2016/09/07: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 27: Line 27:


==Data==
==Data==
* Add data and results here...
<u>stock solutions</u>
# BSA
## 25.33 mg of myoglobin with water in 10.0 mL volumetric flask
## MW = 66776 g/mol
## concentration = 37.93 μM
 
<u>samples</u>
# BSA Fluorescence
## 3 mL total
### 0.25 mM Au
### 3.125 μM BSA
### HCl or NaOH appropriate for your pH
### water
# BSA for UV-Vis during next session
## 5 mL total
### 0.25 mM Au
### 3.125 μM BSA
### HCl or NaOH appropriate for your pH
### Appropriate amount of fructose
### water
# Lysozym Fluorescence
## 3 mL total
### 0.25 mM Au
### 12.5 μM Myoglobin
### HCl or NaOH appropriate for your pH
### water


==Notes==
==Notes==

Revision as of 07:44, 7 September 2016

Biomaterials Design Lab <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>



Objective

Today's schedule

  1. Ocean Optics
    1. Hannah and Cossette
  2. Fluorescence
    1. Sarah, Anneliese, and Patrick
  3. UV-Vis
    1. Cassidy and Sara


Make new Lysozyme stock solution

Description

  1. Add experimental record here. Include what, how, and why...

Data

stock solutions

  1. BSA
    1. 25.33 mg of myoglobin with water in 10.0 mL volumetric flask
    2. MW = 66776 g/mol
    3. concentration = 37.93 μM

samples

  1. BSA Fluorescence
    1. 3 mL total
      1. 0.25 mM Au
      2. 3.125 μM BSA
      3. HCl or NaOH appropriate for your pH
      4. water
  2. BSA for UV-Vis during next session
    1. 5 mL total
      1. 0.25 mM Au
      2. 3.125 μM BSA
      3. HCl or NaOH appropriate for your pH
      4. Appropriate amount of fructose
      5. water
  3. Lysozym Fluorescence
    1. 3 mL total
      1. 0.25 mM Au
      2. 12.5 μM Myoglobin
      3. HCl or NaOH appropriate for your pH
      4. water

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.