User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2016/09/28: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 30: Line 30:
## 300 μL 1mM HCl
## 300 μL 1mM HCl
## 2290 μL water
## 2290 μL water
<u>UV-Vis of sample before and after measurement</u>
[[Image:20160928 mph MyoglobinAuNPUVVis.png]]
<u>Fluorescence data</u>
# Acquisition information
## Excitation: 290 nm, 10 nm slit width
## Emission: 310-540 nm, 10 nm slit width
## 100 nm/minute
## 61 measurements, 180 second delay between measurements
[[Image:20160928 mrh MbAuNP IntegratedI.png]]
[[Image:20160928 mrh MbAuNP EmissionMax.png]]
[[Image:20160928 mrh MbAuNP selectedscans1.png]]
[[Image:20160928 mrh MbAuNP selectedscans2.png]]
[[Image:20160928 mrh MbAuNP selectedscans3.png]]


==Notes==
==Notes==

Revision as of 10:47, 28 September 2016

Biomaterials Design Lab <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>



Objective

Cassidy and Sarah got strange results for their fluorescence measurements yesterday (myoglobin, AuNPs, pH4). So I'm going to re run their samples.

Description

  1. Add experimental record here. Include what, how, and why...

Data

Stock solution for class

  1. AgNO3
    1. 28.13 mg AgNO3
    2. to 10.0 mL in water
    3. 169.87 g/mol
    4. 16.6 mM

Myoglobin Sample Prep

  1. 250 μM AuCl3, 12.5 μM myoglobin, pH 4
    1. 176 μL 4.27 mM AuCl3
    2. 234 μL 160 μM myoglobin
    3. 300 μL 1mM HCl
    4. 2290 μL water


UV-Vis of sample before and after measurement

File:20160928 mph MyoglobinAuNPUVVis.png

Fluorescence data

  1. Acquisition information
    1. Excitation: 290 nm, 10 nm slit width
    2. Emission: 310-540 nm, 10 nm slit width
    3. 100 nm/minute
    4. 61 measurements, 180 second delay between measurements

Notes

Anneliese Faustino




Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2016/09/28&oldid=956244"