User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2016/09/28: Difference between revisions

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<u>UV-Vis of sample before and after measurement</u>
<u>UV-Vis of sample before and after measurement</u>


[[Image:20160928 mph MyoglobinAuNPUVVis.png]]
[[Image:20160928 mrh MyoglobinAuNPUVVis.png]]


<u>Fluorescence data</u>
<u>Fluorescence data</u>

Revision as of 10:48, 28 September 2016

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Objective

Cassidy and Sarah got strange results for their fluorescence measurements yesterday (myoglobin, AuNPs, pH4). So I'm going to re run their samples.

Description

  1. Add experimental record here. Include what, how, and why...

Data

Stock solution for class

  1. AgNO3
    1. 28.13 mg AgNO3
    2. to 10.0 mL in water
    3. 169.87 g/mol
    4. 16.6 mM

Myoglobin Sample Prep

  1. 250 μM AuCl3, 12.5 μM myoglobin, pH 4
    1. 176 μL 4.27 mM AuCl3
    2. 234 μL 160 μM myoglobin
    3. 300 μL 1mM HCl
    4. 2290 μL water


UV-Vis of sample before and after measurement

Fluorescence data

  1. Acquisition information
    1. Excitation: 290 nm, 10 nm slit width
    2. Emission: 310-540 nm, 10 nm slit width
    3. 100 nm/minute
    4. 61 measurements, 180 second delay between measurements

Notes

Anneliese Faustino




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