User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2016/09/30: Difference between revisions

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[[Image:20160930 mrh LysozymepH6 scans.png]]
[[Image:20160930 mrh LysozymepH6 scans.png]]
==Myoglobin Fluorescence==
<u>3 mL Sample</u>
# 12.5 μM BSA
## Stock Concentration 160 μM
## Volume Added: 234 μL
# pH 5
## Stock Concentration 1 mM HCl
## Volume Added: 30 μL
# Volume water added: 2736 μL
<u>UV-Vis of sample</u>
[[Image:20160930 mrh Myoglobin UVVis.png]]
Evidence of protein aggregation (cloudy solution)
<u>Fluorescence scans</u>
# Acquisition information
## Excitation: 290 nm, 10 nm slit width
## Emission: 310-540 nm, 10 nm slit width
## 100 nm/minute
## 61 measurements, 180 second delay between measurements
[[Image:20160930 mrh MyoglobinpH5 IntegratedI.png]]
[[Image:20160930 mrh MyoglobinpH5 EmissionMax.png]]
[[Image:20160930 mrh MyoglobinpH5 scans.png]]


==Data==
==Data==

Revision as of 12:47, 30 September 2016

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Objective

Acquire fluorescence scans of lysozyme (pH 6) and myoglobin (pH 5) at 80C.

Lysozyme Fluorescence

3 mL Sample

  1. 0.5 μM BSA
    1. Stock Concentration 210 μM
    2. Volume Added: 7.1 μL
  2. pH 6
    1. Stock Concentration 1 mM HCl
    2. Volume Added: 3 μL
  3. Volume water added: 2990 μL

UV-Vis of sample

Evidence here of photobleaching of the fluorophore

Fluorescence scans

  1. Acquisition information
    1. Excitation: 290 nm, 10 nm slit width
    2. Emission: 310-540 nm, 10 nm slit width
    3. 100 nm/minute
    4. 61 measurements, 180 second delay between measurements

Myoglobin Fluorescence

3 mL Sample

  1. 12.5 μM BSA
    1. Stock Concentration 160 μM
    2. Volume Added: 234 μL
  2. pH 5
    1. Stock Concentration 1 mM HCl
    2. Volume Added: 30 μL
  3. Volume water added: 2736 μL

UV-Vis of sample

Evidence of protein aggregation (cloudy solution)

Fluorescence scans

  1. Acquisition information
    1. Excitation: 290 nm, 10 nm slit width
    2. Emission: 310-540 nm, 10 nm slit width
    3. 100 nm/minute
    4. 61 measurements, 180 second delay between measurements

Data

  • Add data and results here...

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.




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