User:Matt Hartings/Notebook/Photosynthesis/2012/02/13: Difference between revisions
From OpenWetWare
(Autocreate 2012/02/13 Entry for User:Matt_Hartings/Notebook/Photosynthesis) |
|||
| (One intermediate revision by the same user not shown) | |||
| Line 11: | Line 11: | ||
==Objective== | ==Objective== | ||
Prepping for K31C expression tomorrow. | |||
==Description== | ==Description== | ||
I talked with Kara Bren on Friday. She told me that BL21 (DE3) should produce heme on its own. So, I'm going to attempt expression without adding hemin. | |||
(8am) I autoclaved 2x1L and 2x25ml of LB. | |||
(10:30am) I autoclaved 2x1L and 2x25ml of LB. | |||
(11:30) I prepared 4mL of 100 mg/mL ampicilin in water. | |||
==Data== | ==Data== | ||
Revision as of 10:20, 13 February 2012
 Biomaterials Design Lab
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
|
ObjectivePrepping for K31C expression tomorrow. DescriptionI talked with Kara Bren on Friday. She told me that BL21 (DE3) should produce heme on its own. So, I'm going to attempt expression without adding hemin. (8am) I autoclaved 2x1L and 2x25ml of LB. (10:30am) I autoclaved 2x1L and 2x25ml of LB. (11:30) I prepared 4mL of 100 mg/mL ampicilin in water. Data
NotesThis area is for any observations or conclusions that you would like to note.
| |
