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Prepping for K31C expression tomorrow.
I talked with Kara Bren on Friday. She told me that BL21 (DE3) should produce heme on its own. So, I'm going to attempt expression without adding hemin.
(8am) I autoclaved 2x1L and 2x25ml of LB.
(10:30am) I autoclaved 2x1L and 2x25ml of LB.
(11:30) I prepared 4mL of 100 mg/mL ampicilin in water.
This area is for any observations or conclusions that you would like to note.