User:Matt Hartings/Notebook/Photosynthesis/2012/06/19: Difference between revisions
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==Description== | ==Description== | ||
==Start WT Hemoglobin Expression== | |||
#Inoculate starter cultures (35mL LB, autoclaved) with 35uL of 100mg/mL Ampicilin and cells from WT Ascaris Hemoglobin glycerol stock in -80 freezer. | |||
#Shake/incubate the cultures overnight at 37C | |||
==Continuing Myoglobin Expression== | ==Continuing Myoglobin Expression== | ||
# Spin down cells in starter culture (20 minutes, 4000 rpm, 4C) | # Spin down cells in starter culture (20 minutes, 4000 rpm, 4C) |
Revision as of 11:34, 27 June 2012
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ObjectiveContinue sperm whale myoglobin expression as started by Tamra notebook page and finish purification of protein as started yesterday notebook page. DescriptionStart WT Hemoglobin Expression
Continuing Myoglobin Expression
Purification of WT Ascaris Hemoglobin
Data
NotesThis area is for any observations or conclusions that you would like to note.
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