User:Matt Hartings/Notebook/Photosynthesis/2012/06/28: Difference between revisions
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==Objective== | ==Objective== | ||
Prepare media for hemoglobin expression. | Prepare media for hemoglobin expression and set up starter cultures. | ||
==Procedure== | |||
Starter cultures (x4) | Starter cultures (x4) | ||
# 0.875g LB | # 0.875g LB | ||
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# 1L water | # 1L water | ||
# autoclave | # autoclave | ||
Starter cultures (x4) | |||
# 35uL of 100mg/mL ampicilin was added to each starter culture flask | |||
# Starter culture was inoculated with cells from the BL21(DE3)/pET-3d/WT Ascaris hemoglobin glycerol stock | |||
# Flasks were placed in the shaker/incubator overnight (37C, 250rpm) | |||
Revision as of 12:18, 30 June 2012
 Biomaterials Design Lab
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ObjectivePrepare media for hemoglobin expression and set up starter cultures. ProcedureStarter cultures (x4)
Expression cultures (x4)
Starter cultures (x4)
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