User:Matt Hartings/Notebook/Photosynthesis/2012/06/28

From OpenWetWare
Jump to navigationJump to search
The printable version is no longer supported and may have rendering errors. Please update your browser bookmarks and please use the default browser print function instead.
Biomaterials Design Lab <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>



Objective

Prepare media for hemoglobin expression and set up starter cultures.

Procedure

Starter cultures (x4)

  1. 0.875g LB
  2. 35mL water
  3. autoclave

Expression cultures (x4)

  1. 25g LB
  2. 1L water
  3. autoclave

Starter cultures (x4)

  1. 35uL of 100mg/mL ampicilin was added to each starter culture flask
  2. Starter culture was inoculated with cells from the BL21(DE3)/pET-3d/WT Ascaris hemoglobin glycerol stock
  3. Flasks were placed in the shaker/incubator overnight (37C, 250rpm)



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Matt_Hartings/Notebook/Photosynthesis/2012/06/28&oldid=611338"