User:Matt Hartings/Notebook/Photosynthesis/2012/09/13: Difference between revisions
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|style="background-color: #EEE"|[[Image:Hartings_AU_Photosynthesis_Lab_Header.png|128px]]<span style="font-size:22px;"> Protein Re-engineering</span> | |style="background-color: #EEE"|[[Image:Hartings_AU_Photosynthesis_Lab_Header.png|128px]]<span style="font-size:22px;"> Protein Re-engineering</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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Extract Hb protein from cells. | Extract Hb protein from cells. | ||
==Procedure== | |||
#Thaw cell pellets | |||
#Run 3 cycles of ultrasonication | |||
##30seconds sonicating | |||
##30 seconds on ice | |||
#Balance centrifuge tubes (oak ridge tubes) containing sonicated cells with water | |||
#centrifuge at 18000rpm at 4C for 2 hours | |||
#decant liquid from tubes into fresh falcon tubes. | |||
#store in refrigerator | |||
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Latest revision as of 22:00, 26 September 2017
 Protein Re-engineering
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 Main project page Previous entry Next entry
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ObjectiveExtract Hb protein from cells. Procedure
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