User:Matt Hartings/Notebook/Photosynthesis/2013/03/11: Difference between revisions
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## 0.2005g D-sorbitol | ## 0.2005g D-sorbitol | ||
## 0.110g (1450MW) PEG | ## 0.110g (1450MW) PEG | ||
# 10mL 10mM citrate pH 3 | ## 10mL 10mM citrate pH 3 | ||
# pH 5 | # pH 5 | ||
## 0.0110g Mb | ## 0.0110g Mb | ||
## 0.1946g D-sorbitol | ## 0.1946g D-sorbitol | ||
## 0.100g (1450MW) PEG | ## 0.100g (1450MW) PEG | ||
# 10mL 10mM acetate pH 5 | ## 10mL 10mM acetate pH 5 | ||
# pH 7 | # pH 7 | ||
## 0.020g Mb | ## 0.020g Mb | ||
## 0.1998g D-sorbitol | ## 0.1998g D-sorbitol | ||
## 0.1085g (1450MW) PEG | ## 0.1085g (1450MW) PEG | ||
# 10mL 10mM phosphate pH 7 | ## 10mL 10mM phosphate pH 7 | ||
# pH 9 | # pH 9 | ||
## 0.0118g Mb | ## 0.0118g Mb | ||
## 0.1847g D-sorbitol | ## 0.1847g D-sorbitol | ||
## 0.106g (1450MW) PEG | ## 0.106g (1450MW) PEG | ||
# 10mL 10mM tris pH 9 | ## 10mL 10mM tris pH 9 | ||
#Take these samples and freeze them in liquid nitrogen. | #Take these samples and freeze them in liquid nitrogen. | ||
## All samples are reddish brown except for the acetate, which has a greenish color. | ## All samples are reddish brown except for the acetate, which has a greenish color. | ||
Revision as of 12:38, 12 March 2013
 Protein Re-engineering
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Lyophilizing MbLyophilizing Mb samples for studies in organic solvents
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