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 Protein Re-engineering
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Mb in DMSO
Starting with the lyophilized samples that I made on the 11th I am going to make samples in DMSO with crown or (nBu4)NPF6 made on the 12th
- Starting makeup of sample lyophilized at pH 3
- 0.0115g Mb
- 0.2005g D-sorbitol
- 0.110g (1450MW) PEG
- 10mL 10mM citrate pH 3
- (Sample 1) Measured 3.8mg of solid and added 1mL of DMSO with (nBu4)NPF6
- (Sample 2) Measured 5.8mg of solid and added 1mL of DMSO with 18-crown-6
- Starting makeup of sample lyophilized at pH 5
- 0.0110g Mb
- 0.1946g D-sorbitol
- 0.100g (1450MW) PEG
- 10mL 10mM acetate pH 5
- (Sample 1) Measured 6.2mg of solid and added 1mL of DMSO with (nBu4)NPF6
- (Sample 2) Measured 9.8 mg of solid and added 1mL of DMSO with 18-crown-6
- Starting makeup of sample lyophilized at pH 7
- 0.020g Mb
- 0.1998g D-sorbitol
- 0.1085g (1450MW) PEG
- 10mL 10mM phosphate pH 7
- (Sample 1) Measured 11.6 mg of solid and added 1mL of DMSO with (nBu4)NPF6
- (Sample 2) Measured 12.2 mg of solid and added 1mL of DMSO with 18-crown-6
- Starting makeup of sample lyophilized at pH 9
- 0.0118g Mb
- 0.1847g D-sorbitol
- 0.106g (1450MW) PEG
- 10mL 10mM tris pH 9
- (Sample 1) Measured 6.2 mg of solid and added 1mL of DMSO with (nBu4)NPF6
- (Sample 2) Measured 7.9 mg of solid and added 1mL of DMSO with 18-crown-6
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Protein Re-engineering
