User:Matt Hartings/Notebook/Photosynthesis/2013/04/02: Difference between revisions
From OpenWetWare
(Autocreate 2013/04/02 Entry for User:Matt_Hartings/Notebook/Photosynthesis) |
(fix raw html notebook nav) |
||
(7 intermediate revisions by one other user not shown) | |||
Line 2: | Line 2: | ||
|- | |- | ||
|style="background-color: #EEE"|[[Image:Hartings_AU_Photosynthesis_Lab_Header.png|128px]]<span style="font-size:22px;"> Protein Re-engineering</span> | |style="background-color: #EEE"|[[Image:Hartings_AU_Photosynthesis_Lab_Header.png|128px]]<span style="font-size:22px;"> Protein Re-engineering</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
Line 10: | Line 10: | ||
== | ==Mb Catalysis in Methanol== | ||
Running tests on myoglobin catalysis in methanol today. Catalysis is on the conversion of o-phenylenediamine (OPD) into 2,3-diaminophenazine (DAPN) | |||
[[Image:OPD catalysis reaction.png|500px]]<br> | |||
Spectra of the compounds in the figure below are taken from: Qi et al "Myoglobin as mimetic enzyme and its analytical application in determination of H2O2" Wuhan University Journal of Natural Sciences 2006, <b>11</b>, <i>3</i>, p677 | |||
[[Image:OPDReferenceSpectra.png|300px]]<br> | |||
<u>Stock solutions used</u> | |||
# 16.4mg of o-phenylenediamine in 15.2mL of methanol | |||
# 1.134 ml 30% H2O2 +8.866 ml Methanol | |||
# 8.9mg of (5.7mg myoglobin +0.4861g KCl lyophilized from 20mL of 10mM citrate pH 3) in 1 mL of methanol. | |||
<u>Samples were made by</u> | |||
# 2.7 mL of the OPD solution | |||
# 0.3 mL of the Mb solution | |||
# Start the scan | |||
## 1,000 us integration time | |||
## Average over 500 scans | |||
## Run for 10 minutes (600,000,000 us) | |||
# Add 1mL of the H2O2 solution | |||
## Added after scan reached 1% | |||
# Kinetics scans were run at 5, 15, 25, 35, and 45C. Several before and after spectra were also taken, as were the dark and reference signal spectra. | |||
# Absorbance measurements were calculated using the following equation: A = -log[(SampleSignal-DarkSignal)/(ReferenceSignal-DarkSignal)] | |||
Shown below are kinetics traces and absorbance scans for the different temperatures | |||
[[Image:MRH20130402 5C absorbance.png|350px]] [[Image:MRH20130402 5C Kinetics.png|350px]] <br> | |||
[[Image:MRH20130402 15C absorbance.png|350px]] [[Image:MRH20130402 15C Kinetics.png|350px]] <br> | |||
[[Image:MRH20130402 25C absorbance.png|350px]] [[Image:MRH20130402 25C Kinetics.png|350px]] <br> | |||
[[Image:MRH20130402 35C absorbance.png|350px]] [[Image:MRH20130402 35C Kinetics.png|350px]] <br> | |||
[[Image:MRH20130402 45C absorbance.png|350px]] [[Image:MRH20130402 45C Kinetics.png|350px]] | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> |
Latest revision as of 22:35, 26 September 2017
Protein Re-engineering | Main project page Previous entry Next entry |
Mb Catalysis in MethanolRunning tests on myoglobin catalysis in methanol today. Catalysis is on the conversion of o-phenylenediamine (OPD) into 2,3-diaminophenazine (DAPN)
Stock solutions used
Samples were made by
Shown below are kinetics traces and absorbance scans for the different temperatures |