User:Matt Hartings/Notebook/Photosynthesis/2013/04/02: Difference between revisions
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== | ==Mb Catalysis in Methanol== | ||
Running tests on myoglobin catalysis in methanol today. Catalysis is on the conversion of o-phenylenediamine (OPD) into 2,3-diaminophenazine (DAPN) | |||
[[Image:OPD catalysis reaction.png|500px]]<br> | |||
Spectra of the compounds in the figure below are taken from: Qi et al "Myoglobin as mimetic enzyme and its analytical application in determination of H2O2" Wuhan University Journal of Natural Sciences 2006, <b>11</b>, <i>3</i>, p677 | |||
[[Image:OPDReferenceSpectra.png|300px]]<br> | |||
<u>Stock solutions used</u> | |||
# 16.4mg of o-phenylenediamine in 15.2mL of methanol | |||
# 1.134 ml 30% H2O2 +8.866 ml Methanol | |||
# 8.9mg of (5.7mg myoglobin +0.4861g KCl lyophilized from 20mL of 10mM citrate pH 3) in 1 mL of methanol. | |||
Revision as of 07:14, 3 April 2013
 Protein Re-engineering
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Mb Catalysis in MethanolRunning tests on myoglobin catalysis in methanol today. Catalysis is on the conversion of o-phenylenediamine (OPD) into 2,3-diaminophenazine (DAPN)
Stock solutions used
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