User:Matt Hartings/Notebook/Photosynthesis/2013/04/02

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Mb Catalysis in Methanol

Running tests on myoglobin catalysis in methanol today. Catalysis is on the conversion of o-phenylenediamine (OPD) into 2,3-diaminophenazine (DAPN)

Spectra of the compounds in the figure below are taken from: Qi et al "Myoglobin as mimetic enzyme and its analytical application in determination of H2O2" Wuhan University Journal of Natural Sciences 2006, 11, 3, p677

Stock solutions used

  1. 16.4mg of o-phenylenediamine in 15.2mL of methanol
  2. 1.134 ml 30% H2O2 +8.866 ml Methanol
  3. 8.9mg of (5.7mg myoglobin +0.4861g KCl lyophilized from 20mL of 10mM citrate pH 3) in 1 mL of methanol.

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