User:Matt Hartings/Notebook/Photosynthesis/2013/04/03: Difference between revisions
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== | ==Mb catalysis DMSO== | ||
Following the description from [[User:Matt_Hartings/Notebook/Photosynthesis/2013/04/02|Tuesday]]. | |||
<u>Stock solutions used</u> | |||
# 28.6mg of o-phenylenediamine in 26.5mL of DMSO | |||
# 1.134 ml 30% H2O2 +8.866 ml DMSO | |||
# 11.1mg of (5.7mg myoglobin +0.4861g KCl lyophilized from 20mL of 10mM citrate pH 3) in 1 mL of DMSO. | |||
<u>Samples were made by</u> | |||
# 2.025 mL of the OPD solution | |||
# 0.225 mL of the Mb solution | |||
# Start the scan | |||
## 1,000 us integration time | |||
## Average over 500 scans | |||
## Run for 10 minutes (600,000,000 us) | |||
# Add 0.75mL of the H2O2 solution | |||
## Added after scan reached 1% | |||
# Kinetics scans were run at 25, 35, and 45C (DMSO freezes at around 20C). Several before and after spectra were also taken, as were the dark and reference signal spectra. | |||
# Absorbance measurements were calculated using the following equation: A = -log[(SampleSignal-DarkSignal)/(ReferenceSignal-DarkSignal)] | |||
==Mb Catalysis Isopropanol== | |||
Following the description from [[User:Matt_Hartings/Notebook/Photosynthesis/2013/04/02|Tuesday]]. | |||
<u>Stock solutions used</u> | |||
# 29.2mg of o-phenylenediamine in 27mL of Isopropanol | |||
# 1.134 ml 30% H2O2 +8.866 ml Isopropanol | |||
# 15mg of (5.7mg myoglobin +0.4861g KCl lyophilized from 20mL of 10mM citrate pH 3) in 1.5 mL of isopropanol. | |||
<u>Samples were made by</u> | |||
# 2.025 mL of the OPD solution | |||
# 0.225 mL of the Mb solution | |||
# Start the scan | |||
## 1,000 us integration time | |||
## Average over 500 scans | |||
## Run for 10 minutes (600,000,000 us) | |||
# Add 0.75mL of the H2O2 solution | |||
## Added after scan reached 1% | |||
# Kinetics scans were run at 5, 15, 25, 35, and 45C. Several before and after spectra were also taken, as were the dark and reference signal spectra. | |||
# Absorbance measurements were calculated using the following equation: A = -log[(SampleSignal-DarkSignal)/(ReferenceSignal-DarkSignal)] | |||
All of the kinetics data taken today are no good because I didn't save the dark and reference spectra as Signal files. They were saved as processed absorption spectra. DMSO showed reactivity while there was no reactivity in isopropanol. | |||
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Revision as of 09:23, 4 April 2013
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Mb catalysis DMSOFollowing the description from Tuesday. Stock solutions used
Samples were made by
Mb Catalysis IsopropanolFollowing the description from Tuesday. Stock solutions used
Samples were made by
|