User:Matt Hartings/Notebook/Photosynthesis/2013/05/06: Difference between revisions

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==Objective==
==Mb in MeOH==
Learn how to maintain an OpenWetWare Notebook.


Prepping samples for visit to NIST tomorrow to run Circular Dichroism
# Methanol
## From citrate
### 0.1011g of (5.7mg Mb lyophilized from 20mL of 10mM citrate buffer, pH 3, with 0.4861g KCl)
### 10mL methanol
### Vortex for 2 minutes
### centrifuge at 4500rpm for 15 minutes
## From acetate
### 0.1010g of (3.0mg Mb lyophilized from 20mL of 10mM acetate buffer, pH 5, with 0.4856g KCl)
### 10 mL methanol
### Vortex for 2 minutes
### centrifuge at 4500rpm for 15 minutes
## From phosphate
### 0.1079g of (5.8mg Mb lyophilized from 20mL of 10mM phosphate buffer, pH 7, with 0.4961g KCl)
### 10 mL methanol
### Vortex for 2 minutes
### centrifuge at 4500rpm for 15 minutes
# Water
## From citrate
### 0.0538g of (5.7mg Mb lyophilized from 20mL of 10mM citrate buffer, pH 3, with 0.4861g KCl)
### 5.3mL water
## From acetate
### 0.0525g of (3.0mg Mb lyophilized from 20mL of 10mM acetate buffer, pH 5, with 0.4856g KCl)
### 5 mL water
## From phosphate
### 0.0531g of (5.8mg Mb lyophilized from 20mL of 10mM phosphate buffer, pH 7, with 0.4961g KCl)
### 5 mL water


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Revision as of 11:30, 6 May 2013

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Mb in MeOH

Prepping samples for visit to NIST tomorrow to run Circular Dichroism

  1. Methanol
    1. From citrate
      1. 0.1011g of (5.7mg Mb lyophilized from 20mL of 10mM citrate buffer, pH 3, with 0.4861g KCl)
      2. 10mL methanol
      3. Vortex for 2 minutes
      4. centrifuge at 4500rpm for 15 minutes
    2. From acetate
      1. 0.1010g of (3.0mg Mb lyophilized from 20mL of 10mM acetate buffer, pH 5, with 0.4856g KCl)
      2. 10 mL methanol
      3. Vortex for 2 minutes
      4. centrifuge at 4500rpm for 15 minutes
    3. From phosphate
      1. 0.1079g of (5.8mg Mb lyophilized from 20mL of 10mM phosphate buffer, pH 7, with 0.4961g KCl)
      2. 10 mL methanol
      3. Vortex for 2 minutes
      4. centrifuge at 4500rpm for 15 minutes
  2. Water
    1. From citrate
      1. 0.0538g of (5.7mg Mb lyophilized from 20mL of 10mM citrate buffer, pH 3, with 0.4861g KCl)
      2. 5.3mL water
    2. From acetate
      1. 0.0525g of (3.0mg Mb lyophilized from 20mL of 10mM acetate buffer, pH 5, with 0.4856g KCl)
      2. 5 mL water
    3. From phosphate
      1. 0.0531g of (5.8mg Mb lyophilized from 20mL of 10mM phosphate buffer, pH 7, with 0.4961g KCl)
      2. 5 mL water


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