User:Matt Hartings/Notebook/Photosynthesis/2013/05/06: Difference between revisions
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(Autocreate 2013/05/06 Entry for User:Matt_Hartings/Notebook/Photosynthesis) |
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== | ==Mb in MeOH== | ||
Prepping samples for visit to NIST tomorrow to run Circular Dichroism | |||
# Methanol | |||
## From citrate | |||
### 0.1011g of (5.7mg Mb lyophilized from 20mL of 10mM citrate buffer, pH 3, with 0.4861g KCl) | |||
### 10mL methanol | |||
### Vortex for 2 minutes | |||
### centrifuge at 4500rpm for 15 minutes | |||
## From acetate | |||
### 0.1010g of (3.0mg Mb lyophilized from 20mL of 10mM acetate buffer, pH 5, with 0.4856g KCl) | |||
### 10 mL methanol | |||
### Vortex for 2 minutes | |||
### centrifuge at 4500rpm for 15 minutes | |||
## From phosphate | |||
### 0.1079g of (5.8mg Mb lyophilized from 20mL of 10mM phosphate buffer, pH 7, with 0.4961g KCl) | |||
### 10 mL methanol | |||
### Vortex for 2 minutes | |||
### centrifuge at 4500rpm for 15 minutes | |||
# Water | |||
## From citrate | |||
### 0.0538g of (5.7mg Mb lyophilized from 20mL of 10mM citrate buffer, pH 3, with 0.4861g KCl) | |||
### 5.3mL water | |||
## From acetate | |||
### 0.0525g of (3.0mg Mb lyophilized from 20mL of 10mM acetate buffer, pH 5, with 0.4856g KCl) | |||
### 5 mL water | |||
## From phosphate | |||
### 0.0531g of (5.8mg Mb lyophilized from 20mL of 10mM phosphate buffer, pH 7, with 0.4961g KCl) | |||
### 5 mL water | |||
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Revision as of 11:30, 6 May 2013
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Mb in MeOHPrepping samples for visit to NIST tomorrow to run Circular Dichroism
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