User:Matt Hartings/Notebook/Photosynthesis/2013/06/18

From OpenWetWare

< User:Matt Hartings | Notebook | Photosynthesis | 2013 | 06(Difference between revisions)
Jump to: navigation, search
(Autocreate 2013/06/18 Entry for User:Matt_Hartings/Notebook/Photosynthesis)
Current revision (14:54, 21 June 2013) (view source)
(POM synthesis)
 
(4 intermediate revisions not shown.)
Line 10: Line 10:
-
==Objective==
+
==POM synthesis==
-
Learn how to maintain an OpenWetWare Notebook.
+
Performing the synthesis of Na<sub>12</sub>P<sub>2</sub>W<sub>18</sub>O<sub>56</sub> from K<sub>6</sub>P<sub>2</sub>W<sub>18</sub>O<sub>62</sub> and NaClO<sub>4</sub> as described in [[Hartings_AU_Photosynthesis_Lab:Protocols/Na12P2W18O62 | this]] protocol
 +
Measured out 20.19g K<sub>6</sub>P<sub>2</sub>W<sub>18</sub>O<sub>62</sub> (protocol calls for 20.00g)
 +
Dissolved in 50 mL of water in beaker on stir plate (set to 80C) and cooled to room temperature.
 +
 +
15.409g NaClO<sub>4</sub> added to the solution. A white ppt (KClO<sub>4</sub>) formed immediately.
 +
 +
This solution was allowed to stand for 1 hour. The ppt was filtered out and the green filtrate was retained.
 +
 +
1M Na<sub>2</sub>CO<sub>3</sub> (21.1889g Na<sub>2</sub>CO<sub>3</sub> in 200mL) was added dropwise until the pH reached 9.
 +
 +
The pH was maintained at 9 for an hour.
 +
 +
The product was filtered and the solid washed with 75mL of NaCl saturated water, 75mL of ethanol, and 75mL of diethyl ether.
 +
 +
The solid product was collected and placed in a dessicator (protocol calls for 2 days of dessication over H<sub>2</sub>SO<sub>4</sub>, I am drying it over dririte.)
 +
 +
Notes
 +
# It took a bit of Na<sub>2</sub>CO<sub>3</sub> addition before the solution kept a stable high pH
 +
# The solution started losing its green color and a ppt formed as the pH started to reach a stable value of 8.1
 +
# A pH of 9 was reached after roughly 60mL of 1M Na<sub>2</sub>CO<sub>3</sub> added.
 +
# A total of ~100 mL of 1M Na<sub>2</sub>CO<sub>3</sub> was added through the whole process.
 +
 +
[[User:Matt Hartings|Matt Hartings]] 14:54, 21 June 2013 (EDT) After in the dessicator for two days, the mass was 14.8853g
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
|}
|}
__NOTOC__
__NOTOC__

Current revision

Protein Re-engineering Main project page
Previous entry      Next entry



POM synthesis

Performing the synthesis of Na12P2W18O56 from K6P2W18O62 and NaClO4 as described in this protocol

Measured out 20.19g K6P2W18O62 (protocol calls for 20.00g)

Dissolved in 50 mL of water in beaker on stir plate (set to 80C) and cooled to room temperature.

15.409g NaClO4 added to the solution. A white ppt (KClO4) formed immediately.

This solution was allowed to stand for 1 hour. The ppt was filtered out and the green filtrate was retained.

1M Na2CO3 (21.1889g Na2CO3 in 200mL) was added dropwise until the pH reached 9.

The pH was maintained at 9 for an hour.

The product was filtered and the solid washed with 75mL of NaCl saturated water, 75mL of ethanol, and 75mL of diethyl ether.

The solid product was collected and placed in a dessicator (protocol calls for 2 days of dessication over H2SO4, I am drying it over dririte.)

Notes

  1. It took a bit of Na2CO3 addition before the solution kept a stable high pH
  2. The solution started losing its green color and a ppt formed as the pH started to reach a stable value of 8.1
  3. A pH of 9 was reached after roughly 60mL of 1M Na2CO3 added.
  4. A total of ~100 mL of 1M Na2CO3 was added through the whole process.

Matt Hartings 14:54, 21 June 2013 (EDT) After in the dessicator for two days, the mass was 14.8853g


Personal tools