User:Matt Hartings/Notebook/Photosynthesis/2013/09/27: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(Autocreate 2013/09/27 Entry for User:Matt_Hartings/Notebook/Photosynthesis)
 
Line 11: Line 11:


==Objective==
==Objective==
Learn how to maintain an OpenWetWare Notebook.
Running Mb catalyzed H2O2 and o-phenylenediamine reaction today.
 
==Procedure and Data==
<u>Stock solutions</u>
# Myoglobin
## 2.0mg (Mb:KCl lyophilized from Citrate, 5mg:500mg) in 1.0mL water
# OPD
## 11.5mg OPD in 11.5mL water
# H<sub>2</sub>O<sub>2</sub>
## 0.227mL 30% H<sub>2</sub>O<sub>2</sub> in 1.773mL water
 
<u>Samples 1</u>
# Sample chamber set to 25C and to stir
# 2.025mL OPD
# 0.75mL H<sub>2</sub>O<sub>2</sub>
# Start Data Collection
## 1ms Integration
## 10 scan average
## Wait 1 minute after each scan
## Stop after 2 hours
## Files saved as: Mb_OPD_H2O2_Citrate_H20_SCANNUMBER.txt
# 0.225mL Mb added just before the third scan
## This is denoted in the data file for the third scan




Revision as of 07:58, 27 September 2013

Protein Re-engineering <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>



Objective

Running Mb catalyzed H2O2 and o-phenylenediamine reaction today.

Procedure and Data

Stock solutions

  1. Myoglobin
    1. 2.0mg (Mb:KCl lyophilized from Citrate, 5mg:500mg) in 1.0mL water
  2. OPD
    1. 11.5mg OPD in 11.5mL water
  3. H2O2
    1. 0.227mL 30% H2O2 in 1.773mL water

Samples 1

  1. Sample chamber set to 25C and to stir
  2. 2.025mL OPD
  3. 0.75mL H2O2
  4. Start Data Collection
    1. 1ms Integration
    2. 10 scan average
    3. Wait 1 minute after each scan
    4. Stop after 2 hours
    5. Files saved as: Mb_OPD_H2O2_Citrate_H20_SCANNUMBER.txt
  5. 0.225mL Mb added just before the third scan
    1. This is denoted in the data file for the third scan



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Matt_Hartings/Notebook/Photosynthesis/2013/09/27&oldid=732462"