User:Matt Hartings/Notebook/Photosynthesis/2013/10/03: Difference between revisions
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|style="background-color: #EEE"|[[Image:Hartings_AU_Photosynthesis_Lab_Header.png|128px]]<span style="font-size:22px;"> Protein Re-engineering</span> | |style="background-color: #EEE"|[[Image:Hartings_AU_Photosynthesis_Lab_Header.png|128px]]<span style="font-size:22px;"> Protein Re-engineering</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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### Used the same stock as in sample 2 | ### Used the same stock as in sample 2 | ||
## OPD | ## OPD | ||
### | ### 2.5mg in 2.5mL | ||
## H<sub>2</sub>O<sub>2</sub> | ## H<sub>2</sub>O<sub>2</sub> | ||
### | ### 0.114mL 30% H<sub>2</sub>O<sub>2</sub> in 0.887mL water | ||
#Experimental Run | #Experimental Run | ||
## Sample chamber set to 45C and to stir | ## Sample chamber set to 45C and to stir | ||
Line 80: | Line 80: | ||
### Wait 1 minute after each scan | ### Wait 1 minute after each scan | ||
### Stop after 2 hours | ### Stop after 2 hours | ||
## Files saved as: | ## Files saved as: Mb_OPD_H2O2_Citrate_H20_35_SCANNUMBER.txt [[User:Matt Hartings|Matt Hartings]] I messed up with the naming. Name should have 45 instead of 35. but file says 35 | ||
## 0.225mL Mb stock solution added just before the third scan | ## 0.225mL Mb stock solution added just before the third scan | ||
## This is denoted in the data file for the third scan | ## This is denoted in the data file for the third scan |
Latest revision as of 23:24, 26 September 2017
Protein Re-engineering | Main project page Previous entry Next entry |
ObjectiveTake kinetic data for Mb catalyzed o-phenylenediamine + H2O2 reaction at higher temperatures (25, 35, and 45C) Procedure and DataStock solutions sample 1
sample 2
sample 3
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