User:Matt Hartings/Notebook/Photosynthesis/2013/10/25: Difference between revisions
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# Stock solutions: | # Stock solutions: | ||
## Myoglobin | ## Myoglobin | ||
### 1.4mg (Mb:KCl lyophilized from | ### 1.4mg (Mb:KCl lyophilized from Phosphate, 5mg:500mg) in 1.0mL water | ||
## OPD | ## OPD | ||
### 2.3mg OPD in 2.3mL water | ### 2.3mg OPD in 2.3mL water | ||
Line 34: | Line 34: | ||
### Stop after 2 hours | ### Stop after 2 hours | ||
## Files saved as: Mb_OPD_H2O2_phosphate_water_45_SCANNUMBER.txt | ## Files saved as: Mb_OPD_H2O2_phosphate_water_45_SCANNUMBER.txt | ||
## 0.225mL Mb stock solution added just before the third scan | |||
## This is denoted in the data file for the third scan | |||
<u>sample 1</u> | |||
# Stock solutions: | |||
## Myoglobin | |||
### 1.2mg (Mb:KCl lyophilized from Tris, 5mg:500mg) in 1.0mL water | |||
## OPD | |||
### 3.0mg OPD in 3.0mL water | |||
## H<sub>2</sub>O<sub>2</sub> | |||
### 0.114mL 30% H<sub>2</sub>O<sub>2</sub> in 0.887mL water | |||
#Experimental Run | |||
## Sample chamber set to 45C and to stir | |||
## N<sub>2</sub> run through the cuvette holder to reduce condensation on cuvette | |||
## 2.025mL OPD stock solution | |||
## 0.75mL H<sub>2</sub>O<sub>2</sub> stock solution | |||
### Start Data Collection | |||
### 1ms Integration | |||
### 10 scan average | |||
### Wait 1 minute after each scan | |||
### Stop after 2 hours | |||
## Files saved as: Mb_OPD_H2O2_tris_water_45_SCANNUMBER.txt | |||
## 0.225mL Mb stock solution added just before the third scan | ## 0.225mL Mb stock solution added just before the third scan | ||
## This is denoted in the data file for the third scan | ## This is denoted in the data file for the third scan |
Revision as of 08:03, 25 October 2013
Protein Re-engineering | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveTake kinetic data for Mb (lyophilized from KCl/phosphate buffer) catalyzed o-phenylenediamine + H2O2 reaction in water (45C) and Mb (lyophilized from KCl/tris buffer) catalyzed o-phenylenediamine + H2O2 reaction in water (35, 45C). Procedure and Datasample 1
sample 1
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