User:Matt Hartings/Notebook/Photosynthesis/2013/10/29: Difference between revisions

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==Objective==
==Objective==
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Take kinetic data for Mb (lyophilized from KCl/tris buffer) catalyzed o-phenylenediamine + H2O2 reaction in water (25, 15, and 5C).
 
==Procedure and Data==
 
<u>sample 1</u>
# Stock solutions:
## Myoglobin
### 1.3mg (Mb:KCl lyophilized from Tris, 5mg:500mg) in 1.0mL water
## OPD
### 2.3mg OPD in 2.3mL water
## H<sub>2</sub>O<sub>2</sub>
### 0.114mL 30% H<sub>2</sub>O<sub>2</sub> in 0.887mL water
#Experimental Run
## Sample chamber set to 25C and to stir
## N<sub>2</sub> run through the cuvette holder to reduce condensation on cuvette
## 2.025mL OPD stock solution
## 0.75mL H<sub>2</sub>O<sub>2</sub> stock solution
### Start Data Collection
### 1ms Integration
### 10 scan average
### Wait 1 minute after each scan
### Stop after 2 hours
## Files saved as: Mb_OPD_H2O2_tris_h2o_25_SCANNUMBER.txt
## 0.225mL Mb stock solution added just before the third scan
## This is denoted in the data file for the third scan




Revision as of 05:28, 29 October 2013

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Objective

Take kinetic data for Mb (lyophilized from KCl/tris buffer) catalyzed o-phenylenediamine + H2O2 reaction in water (25, 15, and 5C).

Procedure and Data

sample 1

  1. Stock solutions:
    1. Myoglobin
      1. 1.3mg (Mb:KCl lyophilized from Tris, 5mg:500mg) in 1.0mL water
    2. OPD
      1. 2.3mg OPD in 2.3mL water
    3. H2O2
      1. 0.114mL 30% H2O2 in 0.887mL water
  2. Experimental Run
    1. Sample chamber set to 25C and to stir
    2. N2 run through the cuvette holder to reduce condensation on cuvette
    3. 2.025mL OPD stock solution
    4. 0.75mL H2O2 stock solution
      1. Start Data Collection
      2. 1ms Integration
      3. 10 scan average
      4. Wait 1 minute after each scan
      5. Stop after 2 hours
    5. Files saved as: Mb_OPD_H2O2_tris_h2o_25_SCANNUMBER.txt
    6. 0.225mL Mb stock solution added just before the third scan
    7. This is denoted in the data file for the third scan



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