User:Matt Hartings/Notebook/Photosynthesis/2013/10/29

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Current revision (10:30, 29 October 2013) (view source)
(Procedure and Data)
 
Line 34: Line 34:
### Stop after 2 hours
### Stop after 2 hours
## Files saved as: Mb_OPD_H2O2_tris_h2o_25_SCANNUMBER.txt
## Files saved as: Mb_OPD_H2O2_tris_h2o_25_SCANNUMBER.txt
 +
## 0.225mL Mb stock solution added just before the third scan
 +
## This is denoted in the data file for the third scan
 +
 +
<u>sample 2</u>
 +
# Stock solutions:
 +
## Myoglobin
 +
### same as above
 +
## OPD
 +
### 2.6mg OPD in 2.6mL water
 +
## H<sub>2</sub>O<sub>2</sub>
 +
### 0.114mL 30% H<sub>2</sub>O<sub>2</sub> in 0.887mL water
 +
#Experimental Run
 +
## Sample chamber set to 15C and to stir
 +
## N<sub>2</sub> run through the cuvette holder to reduce condensation on cuvette
 +
## 2.025mL OPD stock solution
 +
## 0.75mL H<sub>2</sub>O<sub>2</sub> stock solution
 +
### Start Data Collection
 +
### 1ms Integration
 +
### 10 scan average
 +
### Wait 1 minute after each scan
 +
### Stop after 2 hours
 +
## Files saved as: Mb_OPD_H2O2_tris_h2o_15_SCANNUMBER.txt
## 0.225mL Mb stock solution added just before the third scan
## 0.225mL Mb stock solution added just before the third scan
## This is denoted in the data file for the third scan
## This is denoted in the data file for the third scan

Current revision

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Objective

Take kinetic data for Mb (lyophilized from KCl/tris buffer) catalyzed o-phenylenediamine + H2O2 reaction in water (25, 15, and 5C).

Procedure and Data

sample 1

  1. Stock solutions:
    1. Myoglobin
      1. 1.3mg (Mb:KCl lyophilized from Tris, 5mg:500mg) in 1.0mL water
    2. OPD
      1. 2.3mg OPD in 2.3mL water
    3. H2O2
      1. 0.114mL 30% H2O2 in 0.887mL water
  2. Experimental Run
    1. Sample chamber set to 25C and to stir
    2. N2 run through the cuvette holder to reduce condensation on cuvette
    3. 2.025mL OPD stock solution
    4. 0.75mL H2O2 stock solution
      1. Start Data Collection
      2. 1ms Integration
      3. 10 scan average
      4. Wait 1 minute after each scan
      5. Stop after 2 hours
    5. Files saved as: Mb_OPD_H2O2_tris_h2o_25_SCANNUMBER.txt
    6. 0.225mL Mb stock solution added just before the third scan
    7. This is denoted in the data file for the third scan

sample 2

  1. Stock solutions:
    1. Myoglobin
      1. same as above
    2. OPD
      1. 2.6mg OPD in 2.6mL water
    3. H2O2
      1. 0.114mL 30% H2O2 in 0.887mL water
  2. Experimental Run
    1. Sample chamber set to 15C and to stir
    2. N2 run through the cuvette holder to reduce condensation on cuvette
    3. 2.025mL OPD stock solution
    4. 0.75mL H2O2 stock solution
      1. Start Data Collection
      2. 1ms Integration
      3. 10 scan average
      4. Wait 1 minute after each scan
      5. Stop after 2 hours
    5. Files saved as: Mb_OPD_H2O2_tris_h2o_15_SCANNUMBER.txt
    6. 0.225mL Mb stock solution added just before the third scan
    7. This is denoted in the data file for the third scan



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