User:Matt Hartings/Notebook/Photosynthesis/2013/11/05: Difference between revisions
From OpenWetWare
(Autocreate 2013/11/05 Entry for User:Matt_Hartings/Notebook/Photosynthesis) |
No edit summary |
||
Line 11: | Line 11: | ||
==Objective== | ==Objective== | ||
Take kinetic data for Mb (lyophilized from KCl/tris buffer) catalyzed o-phenylenediamine + H2O2 reaction in methanol (25, and 35C). | |||
==Procedure and Data== | |||
<u>sample 1</u> | |||
# Stock solutions: | |||
## Myoglobin | |||
### 1.1mg (Mb:KCl lyophilized from Tris, 5mg:500mg) in 1.0mL methanol | |||
## OPD | |||
### 3.0mg OPD in 3.0mL methanol | |||
## H<sub>2</sub>O<sub>2</sub> | |||
### 0.114mL 30% H<sub>2</sub>O<sub>2</sub> in 0.887mL methanol | |||
#Experimental Run | |||
## Sample chamber set to 25C and to stir | |||
## N<sub>2</sub> run through the cuvette holder to reduce condensation on cuvette | |||
## 2.025mL OPD stock solution | |||
## 0.75mL H<sub>2</sub>O<sub>2</sub> stock solution | |||
### Start Data Collection | |||
### 1ms Integration | |||
### 10 scan average | |||
### Wait 1 minute after each scan | |||
### Stop after 2 hours | |||
## Files saved as: Mb_OPD_H2O2_tris_meoh_25_SCANNUMBER.txt | |||
## 0.225mL Mb stock solution added just before the third scan | |||
## This is denoted in the data file for the third scan | |||
<u>sample 2</u> | |||
# Stock solutions: | |||
## Myoglobin | |||
### same as above | |||
## OPD | |||
### 2.5mg OPD in 2.5mL methanol | |||
## H<sub>2</sub>O<sub>2</sub> | |||
### 0.114mL 30% H<sub>2</sub>O<sub>2</sub> in 0.887mL methanol | |||
#Experimental Run | |||
## Sample chamber set to 35C and to stir | |||
## N<sub>2</sub> run through the cuvette holder to reduce condensation on cuvette | |||
## 2.025mL OPD stock solution | |||
## 0.75mL H<sub>2</sub>O<sub>2</sub> stock solution | |||
### Start Data Collection | |||
### 1ms Integration | |||
### 10 scan average | |||
### Wait 1 minute after each scan | |||
### Stop after 2 hours | |||
## Files saved as: Mb_OPD_H2O2_tris_meoh_35_SCANNUMBER.txt | |||
## 0.225mL Mb stock solution added just before the third scan | |||
## This is denoted in the data file for the third scan | |||
Revision as of 11:37, 6 November 2013
Protein Re-engineering | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveTake kinetic data for Mb (lyophilized from KCl/tris buffer) catalyzed o-phenylenediamine + H2O2 reaction in methanol (25, and 35C). Procedure and Datasample 1
sample 2
|