User:Matt Hartings/Notebook/Photosynthesis/2013/11/07: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(Autocreate 2013/11/07 Entry for User:Matt_Hartings/Notebook/Photosynthesis)
 
No edit summary
Line 11: Line 11:


==Objective==
==Objective==
Learn how to maintain an OpenWetWare Notebook.
Take kinetic data for o-phenylenediamine + H2O2 reaction in methanol (25, 35, 45C).  


==Procedure and Data==
<u>sample 1</u>
# Stock solutions:
## Myoglobin
### No Mb used
## OPD
### 2.5mg OPD in 2.5mL methanol
## H<sub>2</sub>O<sub>2</sub>
### 0.114mL 30% H<sub>2</sub>O<sub>2</sub> in 0.887mL methanol
#Experimental Run
## Sample chamber set to 25C and to stir
## N<sub>2</sub> run through the cuvette holder to reduce condensation on cuvette
## 2.025mL OPD stock solution
## 0.75mL H<sub>2</sub>O<sub>2</sub> stock solution
### Start Data Collection
### 1ms Integration
### 10 scan average
### Wait 1 minute after each scan
### Stop after 2 hours
## Files saved as: OPD_H2O2_meoh_25_SCANNUMBER.txt
## 0.225mL methanol
## This is denoted in the data file for the third scan
<u>sample 2</u>
# Stock solutions:
## Myoglobin
### No Mb used
## OPD
### 3.5mg OPD in 3.5mL methanol
## H<sub>2</sub>O<sub>2</sub>
### 0.114mL 30% H<sub>2</sub>O<sub>2</sub> in 0.887mL methanol
#Experimental Run
## Sample chamber set to 35C and to stir
## N<sub>2</sub> run through the cuvette holder to reduce condensation on cuvette
## 2.025mL OPD stock solution
## 0.75mL H<sub>2</sub>O<sub>2</sub> stock solution
### Start Data Collection
### 1ms Integration
### 10 scan average
### Wait 1 minute after each scan
### Stop after 2 hours
## Files saved as: OPD_H2O2_meoh_35_SCANNUMBER.txt
## 0.225mL methanol
## This is denoted in the data file for the third scan


<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Revision as of 08:55, 7 November 2013

Protein Re-engineering <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>



Objective

Take kinetic data for o-phenylenediamine + H2O2 reaction in methanol (25, 35, 45C).

Procedure and Data

sample 1

  1. Stock solutions:
    1. Myoglobin
      1. No Mb used
    2. OPD
      1. 2.5mg OPD in 2.5mL methanol
    3. H2O2
      1. 0.114mL 30% H2O2 in 0.887mL methanol
  2. Experimental Run
    1. Sample chamber set to 25C and to stir
    2. N2 run through the cuvette holder to reduce condensation on cuvette
    3. 2.025mL OPD stock solution
    4. 0.75mL H2O2 stock solution
      1. Start Data Collection
      2. 1ms Integration
      3. 10 scan average
      4. Wait 1 minute after each scan
      5. Stop after 2 hours
    5. Files saved as: OPD_H2O2_meoh_25_SCANNUMBER.txt
    6. 0.225mL methanol
    7. This is denoted in the data file for the third scan

sample 2

  1. Stock solutions:
    1. Myoglobin
      1. No Mb used
    2. OPD
      1. 3.5mg OPD in 3.5mL methanol
    3. H2O2
      1. 0.114mL 30% H2O2 in 0.887mL methanol
  2. Experimental Run
    1. Sample chamber set to 35C and to stir
    2. N2 run through the cuvette holder to reduce condensation on cuvette
    3. 2.025mL OPD stock solution
    4. 0.75mL H2O2 stock solution
      1. Start Data Collection
      2. 1ms Integration
      3. 10 scan average
      4. Wait 1 minute after each scan
      5. Stop after 2 hours
    5. Files saved as: OPD_H2O2_meoh_35_SCANNUMBER.txt
    6. 0.225mL methanol
    7. This is denoted in the data file for the third scan


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Matt_Hartings/Notebook/Photosynthesis/2013/11/07&oldid=755193"