User:Matt Hartings/Notebook/Photosynthesis/2013/11/07

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Current revision (14:05, 7 November 2013) (view source)
(Procedure and Data)
 
Line 56: Line 56:
### Stop after 2 hours
### Stop after 2 hours
## Files saved as: OPD_H2O2_meoh_35_SCANNUMBER.txt
## Files saved as: OPD_H2O2_meoh_35_SCANNUMBER.txt
 +
## 0.225mL methanol
 +
## This is denoted in the data file for the third scan
 +
 +
<u>sample 3</u>
 +
# Stock solutions:
 +
## Myoglobin
 +
### No Mb used
 +
## OPD
 +
### 2.1mg OPD in 2.1mL methanol
 +
## H<sub>2</sub>O<sub>2</sub>
 +
### 0.114mL 30% H<sub>2</sub>O<sub>2</sub> in 0.887mL methanol
 +
#Experimental Run
 +
## Sample chamber set to 45C and to stir
 +
## N<sub>2</sub> run through the cuvette holder to reduce condensation on cuvette
 +
## 2.025mL OPD stock solution
 +
## 0.75mL H<sub>2</sub>O<sub>2</sub> stock solution
 +
### Start Data Collection
 +
### 1ms Integration
 +
### 10 scan average
 +
### Wait 1 minute after each scan
 +
### Stop after 2 hours
 +
## Files saved as: OPD_H2O2_meoh_45_SCANNUMBER.txt
## 0.225mL methanol
## 0.225mL methanol
## This is denoted in the data file for the third scan
## This is denoted in the data file for the third scan

Current revision

Protein Re-engineering Main project page
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Objective

Take kinetic data for o-phenylenediamine + H2O2 reaction in methanol (25, 35, 45C).

Procedure and Data

sample 1

  1. Stock solutions:
    1. Myoglobin
      1. No Mb used
    2. OPD
      1. 2.5mg OPD in 2.5mL methanol
    3. H2O2
      1. 0.114mL 30% H2O2 in 0.887mL methanol
  2. Experimental Run
    1. Sample chamber set to 25C and to stir
    2. N2 run through the cuvette holder to reduce condensation on cuvette
    3. 2.025mL OPD stock solution
    4. 0.75mL H2O2 stock solution
      1. Start Data Collection
      2. 1ms Integration
      3. 10 scan average
      4. Wait 1 minute after each scan
      5. Stop after 2 hours
    5. Files saved as: OPD_H2O2_meoh_25_SCANNUMBER.txt
    6. 0.225mL methanol
    7. This is denoted in the data file for the third scan

sample 2

  1. Stock solutions:
    1. Myoglobin
      1. No Mb used
    2. OPD
      1. 3.5mg OPD in 3.5mL methanol
    3. H2O2
      1. 0.114mL 30% H2O2 in 0.887mL methanol
  2. Experimental Run
    1. Sample chamber set to 35C and to stir
    2. N2 run through the cuvette holder to reduce condensation on cuvette
    3. 2.025mL OPD stock solution
    4. 0.75mL H2O2 stock solution
      1. Start Data Collection
      2. 1ms Integration
      3. 10 scan average
      4. Wait 1 minute after each scan
      5. Stop after 2 hours
    5. Files saved as: OPD_H2O2_meoh_35_SCANNUMBER.txt
    6. 0.225mL methanol
    7. This is denoted in the data file for the third scan

sample 3

  1. Stock solutions:
    1. Myoglobin
      1. No Mb used
    2. OPD
      1. 2.1mg OPD in 2.1mL methanol
    3. H2O2
      1. 0.114mL 30% H2O2 in 0.887mL methanol
  2. Experimental Run
    1. Sample chamber set to 45C and to stir
    2. N2 run through the cuvette holder to reduce condensation on cuvette
    3. 2.025mL OPD stock solution
    4. 0.75mL H2O2 stock solution
      1. Start Data Collection
      2. 1ms Integration
      3. 10 scan average
      4. Wait 1 minute after each scan
      5. Stop after 2 hours
    5. Files saved as: OPD_H2O2_meoh_45_SCANNUMBER.txt
    6. 0.225mL methanol
    7. This is denoted in the data file for the third scan


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