User:Matt Hartings/Notebook/Photosynthesis/2014/09/25: Difference between revisions

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|style="background-color: #EEE"|[[Image:Hartings_AU_Photosynthesis_Lab_Header.png|128px]]<span style="font-size:22px;"> Protein Re-engineering</span>
|style="background-color: #EEE"|[[Image:Hartings_AU_Photosynthesis_Lab_Header.png|128px]]<span style="font-size:22px;"> Protein Re-engineering</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
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# Stock solutions:
# Stock solutions:
## Myoglobin
## Myoglobin
### 15.2mg Mb in 1mL methanol
### 15.2mg Mb lyophilized from KCl/phosphate in 1mL methanol
## OPD
## OPD
### 2.5mg OPD in 2.5mL methanol
### 2.5mg OPD in 2.5mL methanol
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### Stop after 2 hours
### Stop after 2 hours
## Files saved as: OPD_H2O2_meoh_25_SCANNUMBER.txt
## Files saved as: OPD_H2O2_meoh_25_SCANNUMBER.txt
## 0.225mL methanol
## 0.225mL Mb/Methanol mix
## This is denoted in the data file for the third scan
 
<u>sample 2</u>
# Stock solutions:
## KCl Phosphate
### 15.2mg lyophilized KCl/phosphate in 1mL methanol
## OPD
### 2.8mg OPD in 2.8mL methanol
## H<sub>2</sub>O<sub>2</sub>
### 0.114mL 30% H<sub>2</sub>O<sub>2</sub> in 0.887mL methanol
#Experimental Run
## Sample chamber set to 25C and to stir
## N<sub>2</sub> run through the cuvette holder to reduce condensation on cuvette
## 2.025mL OPD stock solution
## 0.75mL H<sub>2</sub>O<sub>2</sub> stock solution
### Start Data Collection
### 1ms Integration
### 10 scan average
### Wait 1 minute after each scan
### Stop after 2 hours
## Files saved as: KCl_OPD_H2O2_meoh_25_SCANNUMBER.txt
## 0.225mL KCl/phosphate in methanol
## This is denoted in the data file for the third scan
## This is denoted in the data file for the third scan



Latest revision as of 00:21, 27 September 2017

Protein Re-engineering Main project page
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Objective

Learn how to maintain an OpenWetWare Notebook.

Samples

sample 1

  1. Stock solutions:
    1. Myoglobin
      1. 15.2mg Mb lyophilized from KCl/phosphate in 1mL methanol
    2. OPD
      1. 2.5mg OPD in 2.5mL methanol
    3. H2O2
      1. 0.114mL 30% H2O2 in 0.887mL methanol
  2. Experimental Run
    1. Sample chamber set to 25C and to stir
    2. N2 run through the cuvette holder to reduce condensation on cuvette
    3. 2.025mL OPD stock solution
    4. 0.75mL H2O2 stock solution
      1. Start Data Collection
      2. 1ms Integration
      3. 10 scan average
      4. Wait 1 minute after each scan
      5. Stop after 2 hours
    5. Files saved as: OPD_H2O2_meoh_25_SCANNUMBER.txt
    6. 0.225mL Mb/Methanol mix
    7. This is denoted in the data file for the third scan

sample 2

  1. Stock solutions:
    1. KCl Phosphate
      1. 15.2mg lyophilized KCl/phosphate in 1mL methanol
    2. OPD
      1. 2.8mg OPD in 2.8mL methanol
    3. H2O2
      1. 0.114mL 30% H2O2 in 0.887mL methanol
  2. Experimental Run
    1. Sample chamber set to 25C and to stir
    2. N2 run through the cuvette holder to reduce condensation on cuvette
    3. 2.025mL OPD stock solution
    4. 0.75mL H2O2 stock solution
      1. Start Data Collection
      2. 1ms Integration
      3. 10 scan average
      4. Wait 1 minute after each scan
      5. Stop after 2 hours
    5. Files saved as: KCl_OPD_H2O2_meoh_25_SCANNUMBER.txt
    6. 0.225mL KCl/phosphate in methanol
    7. This is denoted in the data file for the third scan