User:Matt Hartings/Notebook/Photosynthesis/2014/09/25

From OpenWetWare
Jump to navigationJump to search
Protein Re-engineering <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>



Objective

Learn how to maintain an OpenWetWare Notebook.

Samples

sample 1

  1. Stock solutions:
    1. Myoglobin
      1. 15.2mg Mb lyophilized from KCl/phosphate in 1mL methanol
    2. OPD
      1. 2.5mg OPD in 2.5mL methanol
    3. H2O2
      1. 0.114mL 30% H2O2 in 0.887mL methanol
  2. Experimental Run
    1. Sample chamber set to 25C and to stir
    2. N2 run through the cuvette holder to reduce condensation on cuvette
    3. 2.025mL OPD stock solution
    4. 0.75mL H2O2 stock solution
      1. Start Data Collection
      2. 1ms Integration
      3. 10 scan average
      4. Wait 1 minute after each scan
      5. Stop after 2 hours
    5. Files saved as: OPD_H2O2_meoh_25_SCANNUMBER.txt
    6. 0.225mL Mb/Methanol mix
    7. This is denoted in the data file for the third scan

sample 2

  1. Stock solutions:
    1. KCl Phosphate
      1. 15.2mg lyophilized KCl/phosphate in 1mL methanol
    2. OPD
      1. 2.8mg OPD in 2.8mL methanol
    3. H2O2
      1. 0.114mL 30% H2O2 in 0.887mL methanol
  2. Experimental Run
    1. Sample chamber set to 25C and to stir
    2. N2 run through the cuvette holder to reduce condensation on cuvette
    3. 2.025mL OPD stock solution
    4. 0.75mL H2O2 stock solution
      1. Start Data Collection
      2. 1ms Integration
      3. 10 scan average
      4. Wait 1 minute after each scan
      5. Stop after 2 hours
    5. Files saved as: KCl_OPD_H2O2_meoh_25_SCANNUMBER.txt
    6. 0.225mL KCl/phosphate in methanol
    7. This is denoted in the data file for the third scan


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Matt_Hartings/Notebook/Photosynthesis/2014/09/25&oldid=823745"