User:Matt Hartings/Notebook/Photosynthesis/2015/01/22: Difference between revisions
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|style="background-color: #EEE"|[[Image:Hartings_AU_Photosynthesis_Lab_Header.png|128px]]<span style="font-size:22px;"> Protein Re-engineering</span> | |style="background-color: #EEE"|[[Image:Hartings_AU_Photosynthesis_Lab_Header.png|128px]]<span style="font-size:22px;"> Protein Re-engineering</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==Objective== | ==Objective== | ||
Making a new batch of myoglobin lyophilized from KCl and phosphate | |||
==Procedure== | |||
# Measured out 4.8mg of myoglobin and placed in a 50mL falcon tube | |||
# Measured out 0.498g of KCl and placed in the 50mL falcon tube | |||
# Added 20mL of 10mM phosphate buffer (pH 7) to the falcon tube | |||
# Freezing sample in dry ice (we're out of liquid nitrogen at the moment) | |||
# Add to lyophilizer | |||
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Latest revision as of 00:40, 27 September 2017
Protein Re-engineering | Main project page Previous entry Next entry |
ObjectiveMaking a new batch of myoglobin lyophilized from KCl and phosphate Procedure
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