User:Matthew R Skorski/Notebook/471 - Exp BioChem/2015/09/16: Difference between revisions
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##Tris buffer: 500 mL, pH 9, 10 mM | ##Tris buffer: 500 mL, pH 9, 10 mM | ||
###Used | ###Used | ||
[[User:Matt Hartings|Matt Hartings]] How did you make your buffers? What were the final measurements (conductivity and pH) that you took? Did you have to add HCl/NaOH to either both? What were the calculations/measurements that you used for the phosphate. | |||
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Revision as of 18:09, 22 September 2015
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ObjectiveTo create more AUNP with lysozyme fibers as most of those from 9/15/15 failed to make fibers. Only one 5mL and four 1mL samples generated fibers. The other goal was to make 500 mL of 10 mM Tris buffer, pH 9 and 500 mL of 10 mM phosphate buffer, pH 7. DescriptionThe instructions for the day can be found here.
Matt Hartings How did you make your buffers? What were the final measurements (conductivity and pH) that you took? Did you have to add HCl/NaOH to either both? What were the calculations/measurements that you used for the phosphate. | |
