User:Matthew R Skorski/Notebook/471 - Exp BioChem/2016/03/29: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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Latest revision as of 01:43, 27 September 2017
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ObjectiveToday's objective is to make AuNP fibers using lysozyme and BSA. ProtocolAll of the fibers that I made yesterday formed AuNP. Today, we decided to decrease the amount of lysozyme in our samples in order to encourage better fiber formation. We changed the ratio of lysozyme:gold from 1:45 to 1:60. We also decided to try making fibers using BSA because BSA might also encourage better fiber formation. The ratio of BSA:gold that we used was 1:120. Additionally, we decided to make a batch of 9 lysozyme and 9 BSA samples to incubate in Rhodamine for 2 hours. We also decided to make a batch of 9 lysozyme and 9 BSA samples to incubate in Rhodamine for 24 hours. We made a batch of 7 lysozyme and 7 BSA samples ("blanks") that we will use to make a standard curve for the concentration of Rhodamine as a function of its UV-Vis absorbance. The following reagents we used were as follows:  We made the lysozyme samples as follows:  We were going to make the BSA samples so that they would have a total volume of 10mL, but I screwed up and added too much water, so we made the samples so that they would have a total volume of 12.9mL. We made the BSA samples as follows:  We then incubated the samples at 80 degrees Celsius for 4 hours. Tomorrow, we will incubate samples for 2 and 24 hours. NOTE: We will have to adjust the volume of Rhodamine so that the final concentrations are 10, 5, and 2µM of Rhodamine in the samples for the BSA samples. I factored this in to our spreadsheet. We will also make both standard curves and then measure the absorbance of the 2 hour samples. We will measure the 24 hour samples on Thursday.
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